GMU:DIY Bio: doing things with biology/Nezar Abualhalaweh: Difference between revisions
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The medium was prepared using a magnetic stirrer, in the lab. | The medium was prepared using a magnetic stirrer, in the lab. | ||
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File:Magnetic Stirrer.JPG|Magnetic Stirrer | File: Magnetic Stirrer.JPG|Magnetic Stirrer | ||
</gallery> | </gallery> | ||
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes. | The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes. | ||
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The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes | The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes | ||
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File:P.P Living Sample.JPG|Physarum Polycephalum Sample</gallery> | File:P.P Living Sample.JPG| Physarum Polycephalum Sample</gallery> | ||
Food source: | Food source: | ||
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20 degrees temperature | 20 degrees temperature | ||
Kept in a warm/dark environment. | Kept in a warm/dark environment. | ||
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File:11.Jan.jpeg| P.P Sample 1 (11.Jan) | |||
File:11.Jan2.JPG|P.P Sample 2 (11.Jan) | |||
File:11.Jan3.JPG|P.P Sample 3 (11.Jan) | |||
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Revision as of 19:58, 12 January 2019
Growing Physarum Polycephalum
Medium: 100ml water 3g agar Cooked Directly in a flask on the stove until boiling point, while consistently mixing. The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Sample: The Physarum Polycephalum was taken from healthy dried samples, on a paper and a paper towel.
Food source: Dried Oats (not sterilized)
Conditions: 20 degrees temperature enough moisture (introduced in the initial cultivation) kept in a dark environment
Jan.8.2019
Follow-up on the above Physarum Polycephalum sample in the large Petri dish
Jan.11.2019
Results: The above sample was unsuccessful. Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples.
Growing Physarum Polycephalum from Living Samples
Medium: 200ml water ~2g agar The medium was prepared using a magnetic stirrer, in the lab.
Magnetic Stirrer
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Working Conditions
Sample: The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes
Physarum Polycephalum Sample
Food source: Dried Oats (Sterile) The dried Oats were cooked in the Microwave for ~35 seconds.
Conditions: 20 degrees temperature Kept in a warm/dark environment.
P.P Sample 1 (11.Jan)
P.P Sample 2 (11.Jan)
P.P Sample 3 (11.Jan)
