GMU:DIY Bio: doing things with biology/Nezar Abualhalaweh: Difference between revisions
No edit summary |
No edit summary |
||
| Line 30: | Line 30: | ||
Results: The above sample was unsuccessful. | Results: The above sample was unsuccessful. | ||
Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples. | Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples. | ||
---- | |||
===Growing Physarum Polycephalum from Living Samples=== | |||
Medium: | |||
200ml water | |||
~2g agar | |||
The medium was prepared using a magnetic stirrer, in the lab. | |||
<gallery> | |||
File:Magnetic Stirrer.JPG|Magnetic Stirrer | |||
</gallery> | |||
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes. | |||
Sample: | |||
The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes | |||
Food source: | |||
Dried Oats (Sterile) | |||
The dried Oats were cooked in the Microwave for ~35 seconds. | |||
Conditions: | |||
- 20 degrees temperature | |||
- enough moisture (introduced in the initial cultivation) | |||
- kept in a dark environment | |||
Revision as of 19:39, 12 January 2019
Growing Physarum Polycephalum
Medium: 100ml water 3g agar Cooked Directly in a flask on the stove until boiling point, while consistently mixing. The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Sample: The Physarum Polycephalum was taken from healthy dried samples, on a paper and a paper towel.
Food source: Dried Oats (not sterilized)
Conditions: - 20 degrees temperature - enough moisture (introduced in the initial cultivation) - kept in a dark environment
Jan.8.2019
Follow-up on the above Physarum Polycephalum sample in the large Petri dish
Jan.11.2019
Results: The above sample was unsuccessful. Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples.
Growing Physarum Polycephalum from Living Samples
Medium: 200ml water ~2g agar The medium was prepared using a magnetic stirrer, in the lab.
Magnetic Stirrer
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Sample: The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes
Food source: Dried Oats (Sterile) The dried Oats were cooked in the Microwave for ~35 seconds.
Conditions: - 20 degrees temperature - enough moisture (introduced in the initial cultivation) - kept in a dark environment