GMU:DIY Bio: doing things with biology/Nezar Abualhalaweh: Difference between revisions
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Conditions: | Conditions: | ||
20 degrees temperature | |||
enough moisture (introduced in the initial cultivation) | |||
kept in a dark environment | |||
<gallery> | <gallery> | ||
File:Jan.8.2019.JPG|Jan.8.2019 | File:Jan.8.2019.JPG|Jan.8.2019 | ||
</gallery> | </gallery> | ||
===Follow-up on the above Physarum Polycephalum sample in the large Petri dish=== | ===Follow-up on the above Physarum Polycephalum sample in the large Petri dish=== | ||
<gallery> | <gallery> | ||
File:Jan.11.2019.JPG|Jan.11.2019 | File:Jan.11.2019.JPG|Jan.11.2019 | ||
</gallery> | </gallery> | ||
Results: The above sample was unsuccessful. | Results: The above sample was unsuccessful. | ||
Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples. | Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples. | ||
---- | ---- | ||
===Growing Physarum Polycephalum from Living Samples=== | ===Growing Physarum Polycephalum from Living Samples=== | ||
Medium: | Medium: | ||
200ml water | 200ml water | ||
| Line 38: | Line 34: | ||
The medium was prepared using a magnetic stirrer, in the lab. | The medium was prepared using a magnetic stirrer, in the lab. | ||
<gallery> | <gallery> | ||
File:Magnetic Stirrer.JPG|Magnetic Stirrer | File: Magnetic Stirrer.JPG|Magnetic Stirrer | ||
</gallery> | </gallery> | ||
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes. | The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes. | ||
<gallery> | |||
File:Working_Conditions.JPG|Working Conditions</gallery> | |||
Sample: | Sample: | ||
The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes | The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes | ||
<gallery> | |||
File:P.P Living Sample.JPG| Physarum Polycephalum Sample</gallery> | |||
Food source: | Food source: | ||
| Line 51: | Line 49: | ||
Conditions: | Conditions: | ||
20 degrees temperature | |||
- | Kept in a warm/dark environment. | ||
- | |||
<gallery> | |||
File:11.Jan.jpeg| P.P Sample 1 (11.Jan) | |||
File:11.Jan2.JPG|P.P Sample 2 (11.Jan) | |||
File:11.Jan3.JPG|P.P Sample 3 (11.Jan) | |||
</gallery> | |||
12.Jan - Follow up on the samples above | |||
<gallery> | |||
File:Jan.12.2019(3).jpg| P.P Sample 1 (12.Jan) | |||
File:Jan.12.2019(2).jpg| P.P Sample 2 (12.Jan) | |||
File:Jan.12.2019(1).jpg| P.P Sample 3 (12.Jan) | |||
</gallery> | |||
Results: The above samples were successful, will require follow-up and food change under sterile conditions in the lab. | |||
Latest revision as of 20:07, 12 January 2019
Growing Physarum Polycephalum
Medium: 100ml water 3g agar Cooked Directly in a flask on the stove until boiling point, while consistently mixing. The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Sample: The Physarum Polycephalum was taken from healthy dried samples, on a paper and a paper towel.
Food source: Dried Oats (not sterilized)
Conditions: 20 degrees temperature enough moisture (introduced in the initial cultivation) kept in a dark environment
Jan.8.2019
Follow-up on the above Physarum Polycephalum sample in the large Petri dish
Jan.11.2019
Results: The above sample was unsuccessful. Potential causes: Clear contamination which might be the result of using unsterile oats, and paper/paper towel samples.
Growing Physarum Polycephalum from Living Samples
Medium: 200ml water ~2g agar The medium was prepared using a magnetic stirrer, in the lab.
Magnetic Stirrer
The flask and the large petri dish were both sterilized in the UV chamber for approx. 20 minutes.
Working Conditions
Sample: The Physarum Polycephalum was taken from healthy living samples and transported directly to the Petri dishes
Physarum Polycephalum Sample
Food source: Dried Oats (Sterile) The dried Oats were cooked in the Microwave for ~35 seconds.
Conditions: 20 degrees temperature Kept in a warm/dark environment.
P.P Sample 1 (11.Jan)
P.P Sample 2 (11.Jan)
P.P Sample 3 (11.Jan)
12.Jan - Follow up on the samples above
P.P Sample 1 (12.Jan)
P.P Sample 2 (12.Jan)
P.P Sample 3 (12.Jan)
.jpg)
.jpg)
.jpg)
Results: The above samples were successful, will require follow-up and food change under sterile conditions in the lab.