GMU:BioArt WS16/Freya Probst: Difference between revisions

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==Basic growth medium for bacteria==
==Basic growth medium for bacteria==
 
'''1. Goal:'''
:How can we, as people without access to a professional laboratory, grow microorganisms?  
:How can we, as people without access to a professional laboratory, grow microorganisms?  


'''2. Procedure:'''
'''1. Procedure:'''
:The petri dishes are cleaned with a solution of 70% Alcohol + 30% of water.  
:The petri dishes are cleaned with a solution of 70% Alcohol + 30% of water.  


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Mine contained distilled water, but instead of peptone I used bullion.
Mine contained distilled water, but instead of peptone I used bullion.


'''3. Outcome:'''
'''2. Outcome:'''
:Only the soil sample showed some result. Besides that we can only observe oil drops from the Boullion.
:Only the soil sample showed some result. Besides that we can only observe oil drops from the Boullion.


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:::::[[File:Soil bacteria world2.jpg|550px]]
:::::[[File:Soil bacteria world2.jpg|550px]]
==Bacteria tasting==
:Fermentation is a way of preserving food and the reason for that lies within its sour flavour. Under these sour conditions, neither mushrooms nor bacteria (other than our Lactobacillales) will grow. In our stomachs Lactobacillales takes over important functions and we won't kill it by drinking it. However after taking antibiotics humans can repopulate their gut flora by having a probiotic drinks or foods like the followings.
'''1. Milk -> Yoghurt:'''
    kill possibly pathogenic organisms in the milk: 90°C
    cool milk down to body temperature: 40°C
    add the Lactobacillales (in a few spoons of yoghurt)
:Inside of the incubator the bacteria eat the lactose found in the milk.
'''2. Kambutcha Tea:'''

Revision as of 13:23, 6 November 2016

Basic growth medium for bacteria

How can we, as people without access to a professional laboratory, grow microorganisms?

1. Procedure:

The petri dishes are cleaned with a solution of 70% Alcohol + 30% of water.
   200   ml destilled water/ tapwater
   3 ts  sugar
   2 ts  bouillon/ peptone (proteins)
   2 g   agar (gellant)

We mixed different mediums, varying the ingredients. Tapwater and bouillon are easily accessible, while peptone and destilled water are more specific.

Mine contained distilled water, but instead of peptone I used bullion.

2. Outcome:

Only the soil sample showed some result. Besides that we can only observe oil drops from the Boullion.
Bacteria on boullion.jpg
Soil bacteria world1.jpg
Soil bacteria world2.jpg


Bacteria tasting

Fermentation is a way of preserving food and the reason for that lies within its sour flavour. Under these sour conditions, neither mushrooms nor bacteria (other than our Lactobacillales) will grow. In our stomachs Lactobacillales takes over important functions and we won't kill it by drinking it. However after taking antibiotics humans can repopulate their gut flora by having a probiotic drinks or foods like the followings.

1. Milk -> Yoghurt:

   kill possibly pathogenic organisms in the milk: 90°C
   cool milk down to body temperature: 40°C
   add the Lactobacillales (in a few spoons of yoghurt)
Inside of the incubator the bacteria eat the lactose found in the milk.

2. Kambutcha Tea: