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Sadly, using standard keying software, the diffusion of the medium and the fine veins of Physarum left a big cyan border. Its not unusable but would need a lot of work to be up to standards. | Sadly, using standard keying software, the diffusion of the medium and the fine veins of Physarum left a big cyan border. Its not unusable but would need a lot of work to be up to standards. | ||
This posed the need for a different keying method: The blue channel was extracted from the image and its inverse was used as a luma matte. | This posed the need for a different keying method: The blue channel was extracted from the image and its inverse was used as a luma matte. | ||
[[File:BlueChannel_00000_00000.jpg|400px]] | [[File:BlueChannel_00000_00000.jpg|400px]] | ||
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Previous tests have shown, that a medium that had synthetic ultramarine blue pigment in it, inhibited the growth of physarum. As a workaround, the timelapse-medium had a thick layer of blue medium and on top on that approximately a millimeter of normal, clear medium. With that, a test timelapse was shot overnight (about 10 hours of time). The camera used, was a blackmagic pocket cinema camera 4k, which had the unique advantage of the option to be cable-powered without any accessories. The timelapse was shot in BRAW 12:1 and 3840 by 2160 px and the resulting video was 3 minutes and 16 seconds long. | Previous tests have shown, that a medium that had synthetic ultramarine blue pigment in it, inhibited the growth of physarum. As a workaround, the timelapse-medium had a thick layer of blue medium and on top on that approximately a millimeter of normal, clear medium. With that, a test timelapse was shot overnight (about 10 hours of time). The camera used, was a blackmagic pocket cinema camera 4k, which had the unique advantage of the option to be cable-powered without any accessories. The timelapse was shot in BRAW 12:1 and 3840 by 2160 px and the resulting video was 3 minutes and 16 seconds long. | ||
Because oat flakes in the shot would give away the true scale of the image, a bedding of E. coli bacteria was grown and prepared on the plate. The shot was lit by a continuously running LED-Panel with a power of 15w. | Because oat flakes in the shot would give away the true scale of the image, a bedding of E. coli bacteria was grown and prepared on the plate. The shot was lit by a continuously running LED-Panel with a power of 15w. | ||
Sadly, the specimen of physarum was not very strong and grew only very little during the interval. | Sadly, the specimen of physarum was not very strong and grew only very little during the interval and what grew hat only a very faint yellow tint. | ||
[[File:TimelapseRAW.png|400px]] | |||
This wasted a lot of resolution but might still be usable, depending on the shot size and intended use case. | |||
The cropped and keyed image had a size of roughly 800x800 pixels. | |||
[[File:KeyResult.png|400px]] | |||
To see if it would be usable, a test shot was filmed, 3D Tracked, the footage composited in and graded. The oatflake was masked out to make the slime mold appear larger in size. | |||
In order to hide the bad resolution and make the Physarum more alien-like, a vector blur was applied. |
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