Bureaucrats, emailconfirmed, Administrators
5,734
edits
GMU:DIY Biolab “Driver’s License”/DNA extraction (view source)
Revision as of 15:40, 1 November 2017
, 1 November 2017→DNA
(→DNA) |
|||
| (10 intermediate revisions by the same user not shown) | |||
| Line 56: | Line 56: | ||
*http://www.cohenvanbalen.com/work/sterile | *http://www.cohenvanbalen.com/work/sterile | ||
*http://www.artscatalyst.org/sterile-sensei-ichi-go | *http://www.artscatalyst.org/sterile-sensei-ichi-go | ||
===Joe Davis, Microvenus=== | |||
[[File:microvenus-unmondemoderne.wordpress.jpg|thumb|Fig. 6. Microvenus. Source: http://unmondemoderne.wordpress.com ]] | |||
Microvenus is a genetically modified Escherichia coli bacteria strain carrying a piece of synthetically composed sequence of amino acid molecules. It was first cloned into several laboratory strains of E. coli in collaboration with molecular geneticist Dana Boyd at Jon Beckwith’s laboratory at Harvard Medical School in 1988. As bacteria is small and invisible for the human eye, the artwork instead of being “aesthetic” is rather imaginable. Davis introduces the Microvenus as follow: | |||
"Each Microvenus organism contains many copies of a special molecule designed by the artist and his colleagues. The artistic molecule is a short piece of synthetic DNA containing a coded visual icon that has been incorporated into a living strain of bacteria (E. Coli)."* | |||
The coded icon is a symbol of life from a germanic rune. Resembling letter Y it also represents a female earth in different mythologies. The graphical image was converted into the bit-map image and further into DNA base pairs in order to synthetically program and attach to the original DNA code of the plasmids carried by bacteria (Fig. 6.). Davis has imagined that the code might be interpreted by extraterrestrials as the final stage of the project was supposed to include shipping of the modified bacteria into the universe. | |||
Microvenus is interesting from different perspectives. First of all it is the first genetically modified art work. Secondly, Davis’ idea to encode and store data into the DNA in the end of 80s was a couple of decades ahead of a scientific project presented by CUHK team at the iGEM competition in 2010.* Finally, thinking of extraterrestrial intelligence, it probably makes sense to think of communication happening at the very nature of life, within the chemical interactions between different molecules. So encoding and reading genetical information, might give many answers about different life forms. | |||
*Davis, J. (1996). "Microvenus". Art Journal. 55 (1): 70–74. Available at https://www.jstor.org/stable/777811 | |||
*http://2010.igem.org/Team:Hong_Kong-CUHK | |||
==Terms== | ==Terms== | ||
| Line 76: | Line 89: | ||
“The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix) | “The double-helix model of DNA structure was first published in the journal Nature by James Watson and Francis Crick in 1953”(https://en.wikipedia.org/wiki/Nucleic_acid_double_helix) | ||
<gallery> | |||
File:Wilson1900Fig2.jpg |Onion (Allium) cells in different phases of the cell cycle, drawn by E. B. Wilson, 1900 (wikipedia) | |||
File:Celltypes.png |A eukaryotic cell (left) and a prokaryotic cell (right) (wikipedia) | |||
File:DNA_Structure+Key+Labelled.pn_NoBB.png |The structure of the DNA double helix. The atoms in the structure are colour-coded by element and the detailed structures of two base pairs are shown in the bottom right.(wikipedia) | |||
File:1-dna-polymerase.jpg | |||
File:2-dna-structure.jpg | |||
File:3-what.jpg | |||
File:4-how.jpg | |||
</gallery> | |||
===PCR=== | ===PCR=== | ||
| Line 92: | Line 115: | ||
==DNA extraction== | ==DNA extraction== | ||
https://www.youtube.com/watch?v=7wU97pTs-xE | |||
===Meat tissue cells=== | ===Meat tissue cells=== | ||
*mince thoroughly a small piece of meat tissue | *mince thoroughly a small piece of meat tissue | ||
| Line 101: | Line 126: | ||
===Mouth cells=== | ===Mouth cells=== | ||
*50ml Falkon mit 50ml isotonischer Kochsalzlösung | *50ml Falkon mit 50ml [https://en.wikipedia.org/wiki/Saline_(medicine) isotonischer Kochsalzlösung] | ||
*damit den Mund ordentlich ausspülen (1-2min) | *damit den Mund ordentlich ausspülen (1-2min) | ||
*zurück in den Falkon spucken | *zurück in den Falkon spucken | ||