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GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki (view source)
Revision as of 16:40, 4 October 2010
, 4 October 2010→Notebook
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== Notebook == | == Notebook == | ||
* usage of [http://2009.igem.org/Team:Cambridge E. chromi principle] (pigmented ''E. coli'' cells) as a colorful crowd | * usage of [http://2009.igem.org/Team:Cambridge E. chromi principle] (pigmented ''E. coli'' cells) | ||
** plasmids available in iGEM Spring 2010 DNA Distribution (see table 1) | ** as a colorful crowd | ||
* testing of a synthetic ''E.coli'' predator-prey system ([http://www.nature.com/nchembio/journal/v5/n12/pdf/nchembio.244.pdf Song ''et al.'', 2009]), ([http://www.nature.com/msb/journal/v4/n1/pdf/msb200824.pdf Ballagaddé ''et al.'', 2008]) for final assault on the swarming plate and under the microscope | *** plasmids available in iGEM Spring 2010 DNA Distribution (see table 1) | ||
** kindly provided by R. Smith | * testing of a synthetic ''E.coli'' predator-prey system ([http://www.nature.com/nchembio/journal/v5/n12/pdf/nchembio.244.pdf Song ''et al.'', 2009]), ([http://www.nature.com/msb/journal/v4/n1/pdf/msb200824.pdf Ballagaddé ''et al.'', 2008]) | ||
* wildtype ''E. coli'' for the game-kit | ** for final assault on the swarming plate and under the microscope | ||
** | *** kindly provided by R. Smith | ||
* wildtype ''E. coli'' | |||
** for the game-kit | |||
*** offered by A. Kern | |||
=== | === 30/09/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective LB agar plates (10 g Tryptone, 5 g Yeast extract, 10 g NaCl and 15 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics) | * over-night growth on selective LB agar plates (10 g Tryptone, 5 g Yeast extract, 10 g NaCl and 15 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics) | ||
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| style="font-weight:bold;" | 3a || prey || MG1655 || pLasRLuxI-luxCcdBs, ptet-mCherry(ColE1) || Cm, Kan | | style="font-weight:bold;" | 3a || prey || MG1655 || pLasRLuxI-luxCcdBs, ptet-mCherry(ColE1) || Cm, Kan | ||
|} | |} | ||
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=== | === 31/09/2010 === | ||
* inoculation of day cultures with colonies from the previous day | * inoculation of day cultures with colonies from the previous day | ||
** in 5 mL LB + required antibiotic | ** in 5 mL LB + required antibiotic | ||
* preparation of TB soft agar plates (10 g Tryptone, 5 g NaCl and 3 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics swarm plates) for swarming | * preparation of TB soft agar plates (10 g Tryptone, 5 g NaCl and 3 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics swarm plates) for swarming | ||
** inoculation by carefully pipetting 3 μL of liquid culture into the solidified agar | |||
** incubation of swarm plates at room temperature for 20 h | |||
*** sufficient humidity was ensured by petri dishes filled with water | |||
* inoculation of over-night cultures from the day cultures | |||
=== 01/10/2010 === | |||
* preparation and conduction of microscopy experiments with predator-prey system following [http://www.nature.com/nchembio/journal/v5/n12/extref/nchembio.244-S1.pdf liquid-phase protocols] | |||
== | == | ||