98
edits
GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki (view source)
Revision as of 15:28, 4 October 2010
, 4 October 2010→29/10/2010
| Line 16: | Line 16: | ||
=== 29/10/2010 === | === 29/10/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective agar plates | |||
<br/> | <br/> | ||
| Line 21: | Line 22: | ||
{| class="wikitable sortable" , border="1" | {| class="wikitable sortable" , border="1" | ||
|- bgcolor=grey | |- bgcolor=grey | ||
! height=20px, align='center', width=80px | plasmid ID || width=150px | Part || width=150px | pigment color || width= | ! height=20px, align='center', width=80px | plasmid ID || width=150px | Part || width=150px | pigment color || width=150 px | backbone || width=250 | registry location | ||
|-style="height:20px" | |-style="height:20px" | ||
|+ align="bottom" |''<b>table 1:</b>'' | |+ align="bottom" |''<b>table 1:</b>'' overview of used iGEM constructs | ||
|-align="center" | |-align="center" | ||
| style="font-weight:bold;" | pLA01 || {{Part|BBa_K274110}} || red || {{Part|pSB1A2}} || 2010 Kit Plate 3, 6J | | style="font-weight:bold;" | pLA01 || {{Part|BBa_K274110}} || red || {{Part|pSB1A2}} || 2010 Kit Plate 3, 6J | ||
|-align="center" | |||
| style="font-weight:bold;" | pLA02 || {{Part|BBa_K274210}} || orange || {{Part|pSB1A2}} || 2010 Kit Plate 3, 6N | |||
|-align="center" | |||
| style="font-weight:bold;" | pLA03 || {{Part|BBa_K274002}} || purple || {{Part|pSB1T3}} || 2010 Kit Plate 3, 12B | |||
|-align="center" | |||
| style="font-weight:bold;" | pLA04 || {{Part|BBa_K274003}} || dark green || {{Part|pSB1K3}} || 2010 Kit Plate 3, 20H | |||
|-align="center" | |||
| style="font-weight:bold;" | pLA05 || {{Part|BBa_K274004}} || light green || {{Part|pSB1K3}} || 2010 Kit Plate 3, 20J | |||
|} | |||
<br/> | |||
{| class="wikitable sortable" , border="1" | |||
|- bgcolor=grey | |||
! height=20px, align='center', width=80px | plasmid ID || width=150px | name/function || width=150px | cell strain || width=350 px | plasmid || width=100 | marker | |||
|-style="height:20px" | |||
|+ align="bottom" |''<b>table 1:</b>'' used predator-prey system, gift from R. Smith | |||
|-align="center" | |||
| style="font-weight:bold;" | 1a || predator || MG1655 || ptetLuxRLasI-luxCcdA(SC101) <br/> placCcdBs-tetGFPuv(LVA) || Cm <br/> Kan | |||
|-align="center" | |||
| style="font-weight:bold;" | 3a || prey || MG1655 || pLasRLuxI-luxCcdBs <br/> ptet-mCherry(ColE1) || Cm <br/> Kan | |||
|} | |} | ||