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| ==== '''Day 2 Preparation of Grain Spawn''' ==== | ==== '''Day 2 Preparation of Grain Spawn''' ==== | ||
| Objective: Prepare grain substrate in a jar to produce biomass. | |||
| Materials: pressure cooker, scale, distilled water, rice grains, gypsum (CaSO4 - calcium sulphate), spoon, plate with well-growing mycelium, scalpel, gloves, ethanol, jar, wadding | |||
| <u>Step 1: Weigh Materials</u>  | |||
| {| class="wikitable" | |||
| |+ | |||
| !Component | |||
| !Quantity | |||
| ! | |||
| !Component Quantity Custom Quantity | |||
| |- | |||
| |rice grain | |||
| |30 | |||
| |multiplier | |||
| |g | |||
| |- | |||
| |Calcium sulphate (gypsum)  | |||
| |(2%) 0.6g | |||
| |multiplier | |||
| |g | |||
| |} | |||
| <u>Step 2: Soak rice Grains and Add Gypsum</u>  | |||
| # Pour boiling distilled water over rice grains and allow them to soak for a minimum of one hour to absorb the water fully. | |||
| # Drain any excess liquid. | |||
| # Combine the gypsum with the soaked rice grains, ensuring thorough mixing. | |||
| # Transfer the mixture into a heat-resistant glass jar. | |||
| # Create a ventilation hole in the lid and insert cotton wadding for mushroom air exchange. | |||
| # Seal the jars and cover the lid with aluminum foil. | |||
| <gallery> | |||
| File:Mixed rice 2.jpg | |||
| File:Mixed rice 3.jpg | |||
| File:Mixed rice.jpg | |||
| </gallery><u>Step 3: Sterilize In Pressure Cooker</u> | |||
| <u>Step 4: Add Mycelium</u> | |||
| <u>Step 5: Incubate</u> | |||
| For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] | For reference to the original experiment, please see [https://www.top-ev.de/wp-content/uploads/2021/12/Prepare-rye-jars-v2_1-1.pdf Preparation of Grain Spawn] | ||
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