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Every 3 to 4 weeks, you will need to split the culture in two and put some fresh medium. It is pretty straight forward as you just need a new clean container into which you pour half of your culture in it, and fill the rest with fresh medium. So for a 250ml culture, you would split it in two jar, so 125ml x 2, and then pour 125ml of fresh medium in it. | Every 3 to 4 weeks, you will need to split the culture in two and put some fresh medium. It is pretty straight forward as you just need a new clean container into which you pour half of your culture in it, and fill the rest with fresh medium. So for a 250ml culture, you would split it in two jar, so 125ml x 2, and then pour 125ml of fresh medium in it. | ||
==Documentation and references== | |||
*[https://www.carolina.com/teacher-resources/Interactive/living-organism-care-guide-bioluminescent-dinoflagellates/tr41209.tr Carolina Care Sheet] |
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