GMU:DIY Bio: doing things with biology/Sirin Unmanee/30.10.2018 : Isolating Bacteria from Kombucha, Growing Physarum polycephalum (2nd attempt) and others: Difference between revisions
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There are yellow and orange dots in 1/4 and 1/32 slot, but others remain almost the same, except the medium is covered with icy surface. | |||
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Revision as of 22:23, 7 November 2018
Isolating Bacteria from Kombucha
Here is list of ingredients to prepare 500 ml of liquid Gluconcetobacter medium
Glucose - 10 g
Peptone - 2.5 g
Yeast extract - 2.5 g
Na2HPO4 - 1.35g
Citric acid - 0.75 g
Distilled or tap water - 500 ml
Agar - 7.5 g
However, in this case, I needed just 100 ml, so everything was divided by 5.
I diluted 2 ml of Kombucha and 8 ml of distilled water, then smeared one drop of that solution onto the first slot. The others' solutions were diluted in 1/2, 1/4, 1/8, 1/16 and 1/32 ratio respectively.
30.10.2018 - 20:30
There are yellow and orange dots in 1/4 and 1/32 slot, but others remain almost the same, except the medium is covered with icy surface.
07.11.2018 - 10:30
Growing Physarum polycephalum (2nd attempt)
I failed growing slime mold at my 1st attempt, so I tried once again with three dishes (just in case!), but this time I kept all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing slime mold.
01.11.2018 - 10:30
06.11.2018 - 10:30 under the microcsope
The slime mold is growing quite well.
Growing Bacteria from Chalk
I had Gluconcetobacter left, so I used that medium for growing bacteria from chalk. I expect to have some artworks form it, using a telescope camera.
30.10.2018 - 20:30
07.11.2018 - 10:30