GMU:DIY Bio: doing things with biology/Sirin Unmanee/30.10.2018 : Isolating Bacteria from Kombucha, Growing Physarum polycephalum (2nd attempt) and others: Difference between revisions

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I failed growing slime mold at my 1st attempt, so I tried once again with three dishes (just in case!), but this time I kept all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing slime mold.  
I failed growing slime mold at my 1st attempt, so I tried once again with three dishes (just in case!), but this time I kept all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing slime mold.  
[[File:S__3186890.jpg|200px]][[File:S__3186891.jpg|200px]][[File:S__3186892.jpg|200px]]
<br />''01.11.2018 - 10:30''
I wish I will success growing them this time. Now, after watching them for a while, I have some experimental idea that I want to try.


'''Growing Bacteria from Chalk'''
'''Growing Bacteria from Chalk'''

Revision as of 09:38, 1 November 2018

Isolating Bacteria from Kombucha

Here is list of ingredients to prepare 500 ml of liquid Gluconcetobacter medium

Glucose - 10 g
Peptone - 2.5 g
Yeast extract - 2.5 g
Na2HPO4 - 1.35g
Citric acid - 0.75 g
Distilled or tap water - 500 ml
Agar - 7.5 g

However, in this case, I needed just 100 ml, so everything was divided by 5.

I dissolved 2 ml of Kombucha and 8 ml of distilled water, then smeared one drop of that solution onto the first slot. The others' solutions were dissolved in 1/2, 1/4, 1/8, 1/16 and 1/32 ratio respectively.

S 3178602.jpg
30.10.2018 - 20:30

Growing Physarum polycephalum (2nd attempt)

I failed growing slime mold at my 1st attempt, so I tried once again with three dishes (just in case!), but this time I kept all of them inside the cabinet in the lab. I've learnt form my 1st attempt that my room is not a suitable environment for growing slime mold.

S 3186890.jpgS 3186891.jpgS 3186892.jpg
01.11.2018 - 10:30

I wish I will success growing them this time. Now, after watching them for a while, I have some experimental idea that I want to try.

Growing Bacteria from Chalk

I had Gluconcetobacter left, so I used that medium for growing bacteria from chalk. I expect to have some artworks form it, using a telescope camera.

S 3178597.jpg
30.10.2018 - 20:30