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===Archive=== | |||
Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“. | Take pictures of everything you do, said Miga. But my phone cam was broken. Instead i took this picture with my analogue „exacta135“. | ||
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===Infected by enthusiasm=== | |||
I think the first courseday i really participated. I thought about not even going to the course. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went. | I think the first courseday i really participated. I thought about not even going to the course. And than i met Azuzena in the M18 garden and realized that she was our teacher today. So i went. | ||
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===Media=== | |||
Mycelium grows well in: | Mycelium grows well in: | ||
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31.10. Halloween. | 31.10. Halloween. | ||
===Experiments=== | |||
Motivated by the experiences in the last lesson, i started doing something at home. | |||
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===Analysation of the human DNA by humans=== | |||
We started a Analization of our DNA. Means: gargle a sodium solution and then pipet a lot. We follow the instruction. I think we seperated our DNA... | |||
While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | While a PCR machine redoubles a part of our DNA, marked by a primer. Julian Chollet explains us the process. At first the machine heats up the small Eppis, our DNA double Helix splits up. The primer adopts to a special sequence, on each side one. | ||
„A primer is a short strand of RNA or DNA (generally about 18-22 bases) that serves as a starting point for DNA synthesis. It is required for DNA replication because the enzymes that catalyze this process, DNA polymerases, can only add new nucleotides to an existing strand of DNA. The polymerase starts replication at the 3'-end of the primer, and copies the opposite strand.” (Wikipedia). | |||
Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | Yes and then it cools the Eppendorf test tubes down and the nucleotides can be added to the strand. And this happens a lot of times (30 more or less) until there is enough DNA to be analized. | ||
Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | Only the sequence between the beginning and end primer is being redoubled, that makes diffrent DNA´s comparable. At least parts of it. I think Julian brought us the primer that is used to seperate the part of the Helix that stores the information about our regional origin. | ||
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We wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | We wanted to use gel electrophoresis to compare and analize our DNA, but it didn´t work. We don´t know why. | ||