| Line 337: | Line 337: | ||
*Erlenmeyer flask - high amount of Euglena; fast movement | *Erlenmeyer flask - high amount of Euglena; fast movement | ||
*Beaker glass - moderate amount of Euglena; fast movement | *Beaker glass - moderate amount of Euglena; fast movement | ||
''Day 31'' - checking the vessels: | |||
*Tube 1 - very high amount of Euglena | |||
*Tube 2 - high amount of Euglena; fast movement | |||
*Tube 3 - high amount of Euglena | |||
*Tube 4 - high amount of Euglena; fast movement | |||
*Erlenmeyer flask - high amount of Euglena; fast movement | |||
*Beaker glass - high amount of Euglena; fast movement | |||
After nearly one month of cultivating Euglena the color of all cultures is finally green. Time to sum up the results. | |||
One of the most important things to say is that in every vessel the Euglena increased to a preferable amount, independently from the ingredients I used for the medium. The big difference is the time they needed. | |||
Tube 1 and 2 were very fast because I used the medium I already cooked what means they have had two weeks to grow before I startet Experiment 1. So I was already able to recognize a medium amount of organisms after three days. | |||
Nevertheless I am convinced that the cheese and the fertilizer helped the growing because of some reasons: The concentration was nearly the same in Tube 1 and 2 and in the Erlenmeyer flask after 30 days. That means the growing in the flask speeded up so that in the end it reached the same concentration with a significantly smaller amount of Euglena in the beginning. Also, the tubes only contain 15ml and the Erlenmeyer flask 100ml - another sign that also without the chemicals a high amount of Euglena can be bred in a short time. | |||
Also I guess that it took longer in tube 3 and 4 because they contained the fewest nutrients. I used cheese and fertilizer but the water was distilled. | |||
There was no noticeable difference in the vessels in which I additionally put CO2 fertilizer once a week. | |||
'''My recommendation for growing Euglena:''' | |||
Miga’s recipe (pdf) in combination with a small piece of hard cheese, a warm and light place but no direct sunlight, at least three weeks time and some patience :) | |||
''Note:'' I don’t think the extra solution 3 of the medium is needed anymore because the cheese contains Vitamin B12. To substitute Vitamin B1 some wheat grain should be added as well. I often read that this I used for breeding Euglena too for example [https://www.uni-weimar.de/kunst-und-gestaltung/wiki/images/Culturing-Euglena2.pdf here]. | |||
---- | ---- | ||