GMU:DIY Bio: doing things with biology/Niloofar Ghanavati/Initial Idea: Difference between revisions

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 **Tap water.
 **A plasmodium or sclerotium-available from biological supply houses.
+====Methods:====
+**Cover container bottom with piece of paper towel.
+**Place piece of living plasmodium or sclerotium on paper towel.
+**Moisten paper towel with tap water and drain off excess water.
+**Add one oat flake near slime mold.
+**Place lid over dish and place in a dark place at room temperature (e.g. place in a drawer or closet).
+**Next day examine culture and sprinkle with water to give Physarum a gentle rinse and pour off excess water (rinsing improves growth). Add 1-3 additional oat flakes near advancing front of plasmodium.
+**Continue to examine, rinse, and feed daily or every few days.
+Additional cultures are easily made by transferring a plasmodial covered oat flake to another container. Producing several cultures is desirable to ensure that you don't loose an only culture due to a unforeseen failure of a plasmodium. Also, having more than one culture allows you to test the effects of different treatments on the slime mold (e.g. exposure to light, cold, different food sources, etc.).