GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner: Difference between revisions

GMU:DIY Biolab “Driver’s License”/Jan Georg Glöckner (view source)

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Jan Glöckner

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 they should start to glow in 12-24 hours.
-.November
+'''2.November'''
 Pleurotus growth becomes apparent.
-vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. "(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)"
+vibrio phosp. still no luminescence. maybe the concentration of the the autoinducer is still to low in the medium. ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 42 subchapt. Auto induction)''
+Euglena seem to spin when there is not enough light for them, rather an otherwise. Which makes perfect sense (appetitive behaviour) but somehow I thought it to be the other way round.
+'''8. November'''
+vibrio phosp. dish A shows signs of contamination, I assume due to autoclaving problems as mentioned above.
+still no luminescence, I am beginning to suspect them to be dark mutants ''(Osamu Shimomura: Bioluminescence, Chemical Principles and Methods chapt. 2.7 p. 43 subchapt. Formation of dark mutants)''
+Pleurotus grow is, if, growing very slowly, planning to move on of the two pegs to another medium.
+supplementary: vibrio phosp. opened jar A and shocked it vigorously under the laminar hood, to get oxygen to the bacteria, which should aid glowing. no effects. the sample has to be discarded anyway.
+'''9. November'''
+Discarded jar A of vibrio phosp. due to high contamination.
+transferred on of the pleurotus pegs on malt medium. (Paul Stamets, The Mushroom Cultivator)
+'''10. November'''
+The incubator was switched of. Switched it back on again.
+'''15. November'''
+Pleurotus in malt-medium spawns cottony mycelium. The sample shows mild contamination.
+the tupperware sample still is, if growing very slowly but shows no signs of contamination.
+the temperature of the incubator is now 25.5