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GMU talk:Synthetic Biology/Bacteria Game/iGEM Wiki (view source)
Revision as of 16:03, 4 October 2010
, 4 October 2010→29/10/2010
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=== 29/10/2010 === | === 29/10/2010 === | ||
* [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | * [http://2010.igem.org/Transformation transformation] of TOP10 cells with plasmids (see table 1) out of the registry following [http://partsregistry.org/Help:Spring_2010_DNA_distribution#DNA_Kit_Plate_Instructions standard recommendations] | ||
* over-night growth on selective LB agar plates | * over-night growth on selective LB agar plates (10 g Tryptone, 5 g Yeast extract, 10 g NaCl and 15 g Agar ad 1 L ddH<sub>2</sub>O + required antibiotics) | ||
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* glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective LB agar plates | * glycerol stocks (prepared directly after strains were received) from predator and prey cells (see table 2) were plated on selective LB agar plates (s. a.) | ||
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