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31.10. Halloween. Motivated by the experiences in the last lesson, i started doing something at home. | |||
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File:PA310874.JPG | File:PA310874.JPG | ||
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I think it is contaminated, definetly too wet, says Miga. | I think it is contaminated, definetly too wet, says Miga. | ||
But how can you dry out coffee mold after cooking it (to get rid of contamination) without recontaminating it? | But how can you dry out coffee mold after cooking it (to get rid of contamination) without recontaminating it? | ||
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Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | Well i had a lot of worries. And yes not everything is really working how i thought, but i am still in the process. | ||
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This picture was my phone screen background, it took the same place as a beloved person. | This picture was my phone screen background, it took the same place as a beloved person. | ||
5 days later, it was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | 5 days later, it was already contaminated (the little greenish dots) and began to roll up (in the upper right corner). | ||
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Therefore i cooked some old potatoes | |||
I went to the Lab to make my own PDA-medium. To experiment more with mycelium and find out wich are the best conditions to grow it. Because i think for the paper production i need a lot more mycelium, i needs to be well moisture, for a stable growth, but nor to much medium, so that it can eat up the whole of it and i don´t have to dissolve the medium from the mycelium, because the water is not good for the mycelium either. | |||
Therefore i cooked some old potatoes, and boiled them for maybe 20 minutes, than took them out of the water. Weighed the Agar and the Dextrose, and added them to the Starch- brew. It burned a little on the ground of the pot, because the sugar can´t get too hot. | |||
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File:Potatoes-cooking.JPG | File:Potatoes-cooking.JPG | ||
File:Taking-them-out.JPG | |||
File:Agar&Dextrose.JPG | |||
File:Burned-Pot.JPG | |||
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Meanwhile i had cleaned the "Telstar" disinfected it and a lot of petridishes with alcohol and UV-light. Prepared everything to be ready for the medium. | Meanwhile i had cleaned the "Telstar" disinfected it and a lot of petridishes with alcohol and UV-light. Prepared everything to be ready for the medium. | ||
I filled them and let them cool down with the fan for some minutes. | |||
I waited. | |||
[[File:Filled-Petridishes.JPG|400px]] | [[File:Filled-Petridishes.JPG|400px]] | ||
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This is what you could see 5 Days later: | |||
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I thought, now its proven: the closet is better than the oven! But Maike said, maybe the process in the oven is just fastened and the contamination sleeps in the closet-petridishes as well. So i decided to wait one more week. To see what happens. | I thought, now its proven: the closet is better than the oven! But Maike said, maybe the process in the oven is just fastened and the contamination sleeps in the closet-petridishes as well. So i decided to wait one more week. To see what happens. | ||
One week later was the 28.12.17. I was in YMR for exactly 1 Day, and although i borrowed the key from Tseh, i couldn´t enter the BioLab because the whole building was closed and the Thoska didn´t work. | |||
10.01.2018 | |||
I just took pictures of the petridishes: | |||
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