GMU:DIY Biolab “Driver’s License”/Yang Li: Difference between revisions

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Anthocyanins are water-soluble vacuolar pigments. In the 1664 book Experiments and Considerations Touching Colours by chemist Robert Boyle, various edible plants are reported as visual pH indicators due to pH-responsive mechanisms in their tissues [Boyle, 1664]. Anthocyanin is a kind of natural colorant in food and beverage industry, and has been found to possess anti-inflammatory antioxidant properties [He, 2010]. It has also been researched for use as an indicator for packaging applications to detect spoilage in pork and fish products [Zhang, 2014]. All tissues of vascular plants contain the flavonoid anthocyanin, a pigment that changes colour under varying pH solutions.
Anthocyanins are water-soluble vacuolar pigments. In the 1664 book Experiments and Considerations Touching Colours by chemist Robert Boyle, various edible plants are reported as visual pH indicators due to pH-responsive mechanisms in their tissues [Boyle, 1664]. Anthocyanin is a kind of natural colorant in food and beverage industry, and has been found to possess anti-inflammatory antioxidant properties [He, 2010]. It has also been researched for use as an indicator for packaging applications to detect spoilage in pork and fish products [Zhang, 2014]. All tissues of vascular plants contain the flavonoid anthocyanin, a pigment that changes colour under varying pH solutions.
Under different pH conditions, the hydroxyl (OH) and/or methyl ether (O-CH3) groups attached to the carbon rings (figure 1) undergo reversible structural transformations and ionizations. Restructuring a molecule changes the way it ab- sorbs light, giving rise to colour changes [Kan, 2017].
Under different pH conditions, the hydroxyl (OH) and/or methyl ether (O-CH3) groups attached to the carbon rings (figure 1) undergo reversible structural transformations and ionizations. Restructuring a molecule changes the way it ab- sorbs light, giving rise to colour changes [Kan, 2017].
[[File:Screen Shot 2018-01-31 at 12.08.56.png|400px]]  Figure 1 Chemical diagram of colour-changing anthocyanin pH reaction [UMass Amherst Department of Chemistry ]
 
[[File:Screen Shot 2018-01-31 at 12.08.56.png|400px]]   
Figure 1 Chemical diagram of colour-changing anthocyanin pH reaction [UMass Amherst Department of Chemistry ]


1.2 red cabbage
1.2 red cabbage
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3.2. Making pH solutions
3.2. Making pH solutions
  '''12.12.2017'''
[[File:Screen Shot 2018-01-31 at 15.12.21.png|400px]]
[[File:Screen Shot 2018-01-31 at 15.12.21.png|400px]]
3.3. Process of experiment
3.3. Process of experiment
'''17.1.2018'''
 
  '''17.1.2018'''


However, there’s a problem which I’m not sure:   
However, there’s a problem which I’m not sure:   
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And also, this is a website of Acid/Alkaline Food Chart: http://www.phmiracleliving.com/t-food-chart.aspx
And also, this is a website of Acid/Alkaline Food Chart: http://www.phmiracleliving.com/t-food-chart.aspx
And I find some healthy medicines which are used to adjust pH, the main ingredients are moderately or highly alkaline food.  
And I find some healthy medicines which are used to adjust pH, the main ingredients are moderately or highly alkaline food.  
I am confused with it.


What I will do on 17th:
What I will do on 17th:
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[[File:4591516359873_.pic_hd.jpg|400px]]
[[File:4591516359873_.pic_hd.jpg|400px]]


'''19.1.2018'''
  '''19.1.2018'''


I did the experiment of reversibility of colour change.
I did the experiment of reversibility of colour change.
[[File:Reversibility of Color Change.jpg|400px]]


And then I use reversibility of colour change to make the colour of coffee more obviously.  
And then I use reversibility of colour change to make the colour of coffee more obviously.  
pH of expresso coffee is around 6, I add solution (pH=9) into the original solution 1/2/3, the colour changing is more obviously.
pH of expresso coffee is around 6, I add the solution (pH=8) into the original solution 1/2/3, the colour changing is more obviously.
 
[[File:Reversibility of Color Change.jpg|400px]]


[[File:4581516359845_.pic_hd.jpg|400px]]
[[File:4581516359845_.pic_hd.jpg|400px]]
[[File:result.jpg|400px]]


'''4. Discussion'''


'''4. Discussion'''
[[File:result.jpg|400px]]


'''5. References'''
'''5. References'''
[] Robert Boyle. 1664. Experiments and Considerations Touching Colours. Project Gutenberg. 1–157 pages.
[1] Robert Boyle. 1664. Experiments and Considerations Touching Colours. Project Gutenberg. 1–157 pages.
[] Jian He and M Monica Giusti. 2010. Anthocyanins: Natural Colorants with Health-Promoting Properties.
[2] Jian He and M Monica Giusti. 2010. Anthocyanins: Natural Colorants with Health-Promoting Properties.
[] Xiahong Zhang, Sisi Lu, and Xi Chen. 2014. A visual pH sensing film using natural dyes from Bauhinia blakeana Dunn. Sensors and Actuators B: Chemical 198 (2014), 268–273.
[3] Xiahong Zhang, Sisi Lu, and Xi Chen. 2014. A visual pH sensing film using natural dyes from Bauhinia blakeana Dunn. Sensors and Actuators B: Chemical 198 (2014), 268–273.
[] UMass Amherst Department of Chemistry Lecture Demonstrations.
[4] UMass Amherst Department of Chemistry Lecture Demonstrations.
[] Viirj Kan. 2017. Organic Primitives: Synthesis and Design of pH-Reactive Materials using Molecular I/O for Sensing, Actuation, and Interaction. Proceedings of the 2017 CHI Conference on Human Factors in Computing Systems
[5] Viirj Kan. 2017. Organic Primitives: Synthesis and Design of pH-Reactive Materials using Molecular I/O for Sensing, Actuation, and Interaction. Proceedings of the 2017 CHI Conference on Human Factors in Computing Systems Pages 989-1000.
Pages 989-1000.