Marie EBEL (talk | contribs) No edit summary |
Marie EBEL (talk | contribs) No edit summary |
||
Line 67: | Line 67: | ||
====#First attempt==== | ====#First attempt==== | ||
<gallery> | |||
File:STEP 3.1.JPG|STEP 3.1 | |||
File:STEP 3.2.JPG|STEP 3.2 | |||
File:STEP 3.3.JPG|STEP 3.3 | |||
File:STEP 3.4.jpg|STEP 3.4 | |||
File:STEP 3.5.jpg|STEP 3.5 | |||
File:STEP 3.6.jpg|STEP 3.6 | |||
File:STEP 3.7.JPG|STEP 3.7 | |||
File:STEP 3.8.JPG|STEP 3.8 | |||
File:STEP 3.9.JPG|STEP 3.9 | |||
File:STEP 3.10.JPG|STEP 3.10 | |||
</gallery> | |||
=> I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines). | => I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines). | ||
Line 84: | Line 86: | ||
====#Second attempt==== | ====#Second attempt==== | ||
<gallery> | |||
File:STEP 3.2.1.jpg|STEP 3.2.1 | |||
File:STEP 3.2.2.jpg|STEP 3.2.2 | |||
File:STEP 3.2.3.jpg|STEP 3.2.3 | |||
File:STEP 3.2.4.jpg|STEP 3.2.5 | |||
</gallery> | |||
=> Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | => Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. |