GMU:DIY Biolab “Driver’s License”/Marie Laure Ebel: Difference between revisions

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====#First attempt====
====#First attempt====


[[File:STEP 3.1.JPG|400px]]
<gallery>
[[File:STEP 3.2.JPG|400px]]
File:STEP 3.1.JPG|STEP 3.1
[[File:STEP 3.3.JPG|400px]]
File:STEP 3.2.JPG|STEP 3.2
[[File:STEP 3.4.jpg|400px]]
File:STEP 3.3.JPG|STEP 3.3
[[File:STEP 3.5.jpg|400px]]
File:STEP 3.4.jpg|STEP 3.4
[[File:STEP 3.6.jpg|400px]]
File:STEP 3.5.jpg|STEP 3.5
[[File:STEP 3.7.JPG|400px]]
File:STEP 3.6.jpg|STEP 3.6
[[File:STEP 3.8.JPG|400px]]
File:STEP 3.7.JPG|STEP 3.7
[[File:STEP 3.9.JPG|400px]]
File:STEP 3.8.JPG|STEP 3.8
[[File:STEP 3.10.JPG|400px]]
File:STEP 3.9.JPG|STEP 3.9
File:STEP 3.10.JPG|STEP 3.10
</gallery>


=> I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines).  
=> I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines).  
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====#Second attempt====
====#Second attempt====


[[File:STEP 3.2.1.jpg|400px]]
<gallery>
[[File:STEP 3.2.2.jpg|400px]]
File:STEP 3.2.1.jpg|STEP 3.2.1
[[File:STEP 3.2.3.jpg|400px]]
File:STEP 3.2.2.jpg|STEP 3.2.2
[[File:STEP 3.2.4.jpg|400px]]
File:STEP 3.2.3.jpg|STEP 3.2.3
File:STEP 3.2.4.jpg|STEP 3.2.5
</gallery>


=> Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.  
=> Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.