Marie EBEL (talk | contribs) No edit summary |
Marie EBEL (talk | contribs) No edit summary |
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File:DND test limits.png|DND test limits | File:DND test limits.png|DND test limits | ||
File:DND test limits2.png|DND test limits2 | File:DND test limits2.png|DND test limits2 | ||
File:DND testing sofware.png|DND testing | File:DND testing sofware.png|DND testing software | ||
</gallery> | </gallery> | ||
After making myself familiar with this new terminology, I have been gathering different protocols which would allow me to identify a certain percentage of Asian patterns in my DNA sequences. But unfortunately, I found out that it was more complex than I first thought, for multiple reasons. The first is the fact that those protocols required a more specific equipment (than PCR machine), but then and above all, an access to a DNA database is indispensable. Indeed, the overall principle of each method is about comparing DNA samples with an already defined categorized population guide. In addition, the amount of genetic variations among different population group is 10%-20% only, and within a population component of genetic variation of 93% to 95% has been established. | |||
Thus, categorizing a group of population is already a long, complex and tricky method, but then, finding fragments of different ethnicity patterns within a single DNA sample turns towards a speculative work. Plus, even if I would have found some significant Asian genetic patterns in my blood, those very patterns would have been compared with actual Asian population, and not with an ancestral one (which could differ) | |||
==PRACTICING PCR ANALYSIS== | ==PRACTICING PCR ANALYSIS== | ||
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[[File:STEP 3.10.JPG|400px]] | [[File:STEP 3.10.JPG|400px]] | ||
=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | => I got no results: either the ladder nor the DNA samples were "growing" as they should (multiple lines). | ||
So I decided to test the ladder only to see if there is a problem | So I decided to test the ladder only to see if there is a problem with the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | ||
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[[File:STEP 3.2.4.jpg|400px]] | [[File:STEP 3.2.4.jpg|400px]] | ||
=> Same problem : the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem | => Same problem: the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem since the previous tube was quite old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. | ||
So I decided to test if this | So I decided to test if this blue color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt | ||
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[[File:STEP 3.3.2.jpg|400px]] | [[File:STEP 3.3.2.jpg|400px]] | ||
=> Still turning | => Still turning blue. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water) | ||
So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | ||
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[[File:STEP 3.4.2.jpg|400px]] | [[File:STEP 3.4.2.jpg|400px]] | ||
=> First picture : distilled water only. Second picture : distilled water + buffer TAE 50x. No changes at all. | => First picture: distilled water only. Second picture: distilled water + buffer TAE 50x. No changes at all. | ||
So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect | So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect the electrophoresis itself. | ||