GMU:DIY Biolab “Driver’s License”/Marie Laure Ebel: Difference between revisions

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 [[File:STEP 3.2.4.jpg|400px]]
-=> Same problem : the ladder was growing as one bloc only (see the little drawing) + we noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.
+=> Same problem : the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem, since the previous tube was quiet old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down.
-So we decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt
+So I decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt
 #Third attempt
+[[File:STEP 3.3.1.jpg|400px]]
+[[File:STEP 3.3.2.jpg|400px]]
+=> Still turning bleu. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water)
+So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt
+#Fourth attempt
+[[File:STEP 3.4.1.jpg|400px]]
+[[File:STEP 3.4.2.jpg|400px]]
+=> First picture : distilled water only. Second picture : distilled water + buffer TAE 50x. No changes at all.
+So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect on the electrophoresis itself.