GMU:BioArt WS15/Sebastian Kaye: Difference between revisions

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[[File:PCR_Side view_wiki.jpg]]

A finished PRC Machine : one of three

With the 3 PCR's assembled and ready on Friday evening, Saturday morning came where we followed a DNA extraction protocol using the solutions (Primers.. Master mix.. Buffers.ec.t..) which came from a PRC Kit.

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[[File:PCR_cycles_running.jpg]]

PCR Starts its first cycle!

The PCR Starts its first cycle!

1. 95 °C Denaturation step. First, you heat the DNA to a high temperature (95 °C) so that the two strands of genomic DNA, and later PCR DNA, separate.

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3. Extension Step. Third, you raise the temperature to about 70 °C to activate a DNA polymerase and elongate the primer with respect to the template strand.

This sequence above is repeated about 35 times. This results in 34,359,738,368 copies of the starting DNA sample.

After the first three cycles, we will have the first perfect copy.

POST PCR...

There are several Methods for analysing DNA. Here we are making a gel Chamber for electrophoresis.

This basically is running an a electrical current though some gel. One positive cathode at one end, and the the Negative at the other.

By setting the gel with a 'comb' you create small pockets where the DNA samples can be added along with a tracer.

[[File:Combs_gel_Chamber.jpg]]

The combs in place, ready for the gel to be added..

[[File:Filling_Gel-Chamber.jpg]]

Filling the Chamber with gel. Then leaving it to set, before adding the Traces with Amplified DNA.

My Phone died at this point and some I have no more footage.

@@ Line 56: / Line 56: @@
 [[File:PCR_Side view_wiki.jpg]]
+A finished PRC Machine : one of three
 With the 3 PCR's  assembled and ready on Friday evening, Saturday morning came where we followed a DNA extraction protocol using the solutions (Primers.. Master mix.. Buffers.ec.t..) which came from a PRC Kit.
@@ Line 95: / Line 96: @@
 [[File:PCR_cycles_running.jpg]]
-PCR Starts its first cycle!
+The PCR Starts its first cycle!
 . 95 °C Denaturation step. First, you heat the DNA to a high temperature (95 °C) so that the two strands of genomic DNA, and later PCR DNA, separate.
@@ Line 103: / Line 104: @@
 . Extension Step. Third, you raise the temperature to about 70 °C to activate a DNA polymerase and elongate the primer with respect to the template strand.
 This sequence above is repeated about 35 times. This results in 34,359,738,368 copies of the starting DNA sample.
+After the first three cycles, we will have the first perfect copy.
+POST PCR...
+There are several Methods for analysing DNA. Here we are making a gel Chamber for electrophoresis.
+This basically is running an a electrical current though some gel. One positive cathode at one end, and the the Negative at the other.
+By setting the gel with a 'comb' you create small pockets where the DNA samples can be added along with a tracer.
+[[File:Combs_gel_Chamber.jpg]]
+The combs in place, ready for the gel to be added..
+[[File:Filling_Gel-Chamber.jpg]]
+Filling the Chamber with gel. Then leaving it to set, before adding the Traces with Amplified DNA.
+My Phone died at this point and some I have no more footage.