No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
== Project: Euglena== | |||
[[File:Konsultation and my own ideas_230117_113402_1.jpg|400px]] | |||
[[File:Konsultation and my own ideas_230117_113402_2.jpg|400px]] | |||
[[File:Konsultation and my own ideas_230117_113402_3.jpg|400px]] | |||
[[File:Konsultation and my own ideas_230117_113402_4.jpg|400px]] | |||
[[File:Konsultation and my own ideas_230117_113402_5.jpg|400px]] | |||
=== To-Do's === | |||
*grow Euglena | |||
* care for Euglena | |||
* work out a creative Projekt using Euglena | |||
=== Process of growing Euglena: === | |||
16th of November: start growing Euglena gracilis. | |||
=== Making the substrate === | |||
For the substrate we followed this guide. | |||
[[File:Euglena_sheet.jpg|300px]] | |||
We decided to make 500ml of the solution so we could have a few tries from the beginning in case some of them failed. Working with live organisms is a lot of trial and error. | |||
For that I first did some calculations and multiplied all the necessary ingredient times five, since the recipe was originally for 100ml of solution. I wrote everything down to make it easier for myself once I actually got to weigh everything with the scales. | |||
[[File:notebookentry.jpg|300px]] | |||
I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | |||
I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | |||
I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker. | |||
After preparing the solutions in their respective bottles we put them in the pressure cooker to kill off any spores or bacteria that might have landed in them while working (Make sure the lid of the bottle is on the bottle but not screwed all the way shut to prevent the glass from breaking in the pressure cooker. Also make sure somebody is there to watch the pressure cooker for workplace safety. Alessandro kindly did that for us while we went and got lunch). | |||
Once everything was nice and sterile we prepared our workbench for making the actual substrate and put some Euglena in there for growing. We prepared all the solutions and put them where I can easily reach them. I also got the worksheet ready so I know how much has to go in the base solution, the vitamin solution and the Euglena sample to make sure I can work quickly and efficiently without having to search for things when I'm already working on it. Then I desinfected my hands, put gloves on and desinfect them again. Since we made more of the base solution I also had to take that into account when dropping in the other solutions. After the full substrate was done I filled four plastic containers until about 3/4 full and added a few of the drops with Euglena in them. Then we put all the tubes in a stand near a window to make sure the Euglena could photosynthesize and grow. Since it's winter Alessandro put up a UV lamp for them. | |||
<gallery> | |||
File:IMG_20221116_163456.jpg | |||
File:IMG_20221116_163505.jpg | |||
File:IMG_20221116_163506.jpg | |||
File:IMG_20221116_163512.jpg | |||
File:IMG_20221116_163518.jpg | |||
File:IMG_20221116_163527.jpg | |||
File:IMG_20221116_163528.jpg | |||
File:IMG_20221116_163531.jpg | |||
File:IMG_20221116_163533.jpg | |||
File:IMG_20221116_163535.jpg | |||
File:IMG_20221116_163537.jpg | |||
File:IMG_20221116_163540.jpg | |||
File:IMG_20221116_163544.jpg | |||
File:IMG_20221116_163555.jpg | |||
File:IMG_20221116_163603.jpg | |||
File:IMG_20221116_163605.jpg | |||
File:IMG_20221116_163607.jpg | |||
File:IMG_20221116_163630.jpg | |||
File:IMG_20221116_163634.jpg | |||
File:IMG_20221116_163734.jpg | |||
File:IMG_20221116_163814.jpg | |||
File:IMG_20221116_163810.jpg | |||
File:IMG_20221116_163843.jpg | |||
File:IMG_20221116_163928.jpg | |||
File:IMG_20221116_163931.jpg | |||
File:IMG_20221116_163934.jpg | |||
File:preparing euglena 1.jpg | |||
File:preparing euglena2.jpg | |||
File:preparing euglena3.jpg | |||
File:preparing euglena4.jpg | |||
File:preparing euglena5.jpg | |||
File:preparing euglena6.jpg | |||
File:preparing euglena7.jpg | |||
File:preparing euglena8.jpg | |||
File:preparing euglena9.jpg | |||
File:preparing euglena10.jpg | |||
File:preparing euglena11.jpg | |||
File:preparing euglena12.jpg | |||
</gallery> | |||
=== Footage of the grown Euglena === | |||
<gallery> | |||
File:Euglena_water_rim.JPG | |||
File:Euglenadrop.JPG | |||
File:Euglena_mist.JPG | |||
File:Euglena2.JPG | |||
File:Euglena1.JPG | |||
</gallery> | |||
<videoflash type="vimeo">795559259|437|236</videoflash> | |||
=== Problems/Difficulties that occured during the process === | |||
The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving. | |||
Second thing was that I had a few problems with taking photos and videos from the microscope since I didn't really know how to make the camera take fluid and good quality videos. It was a little frustrating to look into the microscope and see so much color and life in the euglena only to see that the camera can't really pick up on these images as well as I'd hoped. | |||
In general I noticed that one semester is way too short to grow organisms and also have enough time in the end to produce a satisfying art project with them. So I hope I'll get the opportunity to try again when there's more time and not a praxissemester coming up. | |||
== Documenation of the main Workshop 04.11.22 == | == Documenation of the main Workshop 04.11.22 == | ||
| Line 194: | Line 296: | ||
* when does it glow? How does it decide to glow? Can it be used during the day? | * when does it glow? How does it decide to glow? Can it be used during the day? | ||
* examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases | * examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases | ||