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* examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases | * examine exit signs; Are there brightnesslevels that have to be achieved? Is the case a suitable home for the mushroom? Who to ask for permission to install the mushroom cases | ||
== | == Project: Euglena== | ||
=== To-Do's === | |||
== To-Do's == | |||
*grow Euglena | *grow Euglena | ||
* care for Euglena | * care for Euglena | ||
* work out a creative Projekt using Euglena | * work out a creative Projekt using Euglena | ||
== Process of growing Euglena: == | === Process of growing Euglena: === | ||
I met with Alessandro on the 16th of November to start growing Euglena gracilis. | I met with Alessandro on the 16th of November to start growing Euglena gracilis. | ||
== Making the substrate == | === Making the substrate === | ||
For the substrate we followed this guide. | For the substrate we followed this guide. | ||
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I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | I got into the lab equipment which consists of a lab coat, gloves and goggles. I sterilised my hands before and after getting on the gloves, as well as my workspace and all my materials to minimize contaminations that could jeopardize growing the culture of Euglena. | ||
I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | I prepped my workspace with the necessary tools: a highly accurate scale (for the tiny amounts of chemicals you need), the necessary chemicals in order of the recipe, distilled water, a tiny spatula, tin foil as a base to put the chemicals on for weighing, my bottle for the finished solution; sanitizer and papertowels to clean the spatula between going into the different chemicals to prevent crosscontamination of products. | ||
I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker. | I made the base solution and solution one with the help of Alessandro. Solution 2 and 3 were kindly provided by Miga. In the base solution I then dropped the extra solutions 1 and 2 as specified in the text (5 times 8 drops for each) before they go to be autoclaved in the pressure cooker. | ||
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</gallery> | </gallery> | ||
== Ideas for using Euglena == | === Ideas for using Euglena === | ||
[[File:Konsultation and my own ideas_230117_113402_1.jpg|400px]] | [[File:Konsultation and my own ideas_230117_113402_1.jpg|400px]] | ||
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[[File:Konsultation and my own ideas_230117_113402_5.jpg|400px]] | [[File:Konsultation and my own ideas_230117_113402_5.jpg|400px]] | ||
== Footage of the grown Euglena == | === Footage of the grown Euglena === | ||
<gallery> | <gallery> | ||
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<videoflash type="vimeo">795559259|437|236</videoflash> | <videoflash type="vimeo">795559259|437|236</videoflash> | ||
== Problems/Difficulties that occured during the process == | === Problems/Difficulties that occured during the process === | ||
The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving. | The first thing that happened that didn't turn out to be too big of a deal was that only one of the four tubes with Euglena that we made was actually usable. The others had contaminations and/or didn't grow. Which is a good reminder that these things can happen when you are working with living beings. So always have a backup and check in regularly to make sure they're still thriving. | ||