GMU:If the organism will not come to me, I will go to the organism/Kristin Jakubek: Difference between revisions
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[[File:Physarum04.png|400px]] | [[File:Physarum04.png|400px]] | ||
Sun. 31.05 | |||
Starting to notice this white-ish slime. First I thought it was mold but now I think it is the trace that Physarum leaves behind itself - which if it has enough space hinders it from going to this spot again. Might be having a strange reaction with the Agar? | |||
[[File:Physarum05.png|400px]] | [[File:Physarum05.png|400px]] | ||
[[File:Physarum06.png|400px]] | [[File:Physarum06.png|400px]] | ||
Sun. 31.05 | |||
Seeing an answer to the question: does physarum grow on surfaces that are not Agar: yes! | |||
Does it maybe draw the moisture from the surface of agar so that it doesn't need it in other/all areas? | |||
Also you can start to see the digested oats that are no longer yellow so the blob moved off of them because they offer no more for it. | |||
[[File:Physarum07.png|400px]] | [[File:Physarum07.png|400px]] | ||
Sat. 30.05 - decided to 'feed' some less to start seeing a difference in behaviour | |||
Sun. 31.05 - because I don't want to much moisture to build up (might lead to bacterial mold) and to keep caring well for the Physarum & first get a better understanding of it, I gave each dish a full spoon of oats today. | |||
[[File:Physarum08.png|400px]] | [[File:Physarum08.png|400px]] | ||
[[File:Physarum09.png|400px]] | [[File:Physarum09.png|400px]] | ||
The Physarum's structure is obviously much better visible when NOT ON OATS - so what are aesthetic and sustainable ways to replace the oats or make the food source disappear? | |||
[[File:Physarum10.png|400px]] | [[File:Physarum10.png|400px]] | ||
[[File:Physarum11.png]] | [[File:Physarum11.png]] | ||
Mon. 01.06 - The first petri-dish, probably older than a week now changed colors, it doesn't look like mold but it's clearly much much darker then if was yesterday. | |||
Also doesn't look like the other phases of the life cycle? | |||
Mon. 01.06 - Also there is a lot more moisture in the dishes again - maybe today is finally a day to inoculate? | |||
Clearly 6 days is the limit - better to change them after 5 days - like this guide also suggests: https://drive.google.com/drive/folders/1BYdpvPxnUNgdqqu1ELHTnbWlc0cQqvu6 | |||
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Mon. 01.06 - Here you can see Physarum build a squiggly mass on top of the oats, it's more like a pile of yellow little blobs than the vein like structure. | |||
[[File:Physarum14.png]] | [[File:Physarum14.png]] | ||
Mon. 01.06 - Also other dishes are starting to form black dots which I can see more clearly from the bottom - which show bacterial/Yeast contamination and indicate that I should change the dish. | |||
Clearly the Physarum wants out, but isen't even healthy enough to make it. | |||
A new inoculation follows! | |||
What isen't really clear to me is what to do with the old dishes from which I inoculate new ones? Do I let them go their course? Do I try to transfer as much of the blob and then let the leftovers dry? | |||
Answer - Mon. 08.06: I wait until the dish is fully dried out our possibly contaminated to throw away whole dish in worst case or clean out the already dried Physarum. I now have pre-made petri-dishes for when a lot tries to escape. If only a little tries to come out I put it back in the same dish. | |||
[[File:Physarum15.png]] | [[File:Physarum15.png]] | ||