Paola S. Calderón/Specimen adoption 1: ''Haematococcus Pluvialis''.: Difference between revisions
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After the attempt of cultivation of this specimen, I get into the conclusion that the environmental conditions for the Haematococcus-pluvialis were not ideal, in order for it to get out of its hibernation state. My first hypothesis is that it didn't have the ideal conditions in terms of light, air, and temperature, and my second hypothesis is that it didn't have the ideal medium (lack of vinasse in the medium). | After the attempt of cultivation of this specimen, I get into the conclusion that the environmental conditions for the Haematococcus-pluvialis were not ideal, in order for it to get out of its hibernation state. My first hypothesis is that it didn't have the ideal conditions in terms of light, air, and temperature, and my second hypothesis is that it didn't have the ideal medium (lack of vinasse in the medium). | ||
'''Medium''' | '''Medium''' | ||
- 100ml of water | - 100ml of water. | ||
- 3% of vinasse | - 3% of vinasse. | ||
- 0.7% of NaCl. | - 0.7% of NaCl. | ||
The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture. | The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture. | ||
'''Diary of work.''' | '''Diary of work.''' | ||