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==Project Ideas==
'''Project Ideas'''


*analog animation
*analog animation
Line 16: Line 15:




'''First thought'''
===First thought===


Jazz its difficult to define cause contains many sub-genres and improvisation its one of its key elements.         
Jazz its difficult to define cause contains many sub-genres and improvisation its one of its key elements.         
Line 23: Line 22:




'''Second thought'''
===Second thought===


Jazz is essentially a improvisation musik style. It evoques the myth of resistance.  Jazz focus on individual freedom and an authentic expression of oneself, as well as the concept of living in the present.  
Jazz is essentially a improvisation musik style. It evoques the myth of resistance.  Jazz focus on individual freedom and an authentic expression of oneself, as well as the concept of living in the present.  
Line 30: Line 29:




'''Third thought'''
===Third thought===


Jazz interpretation involves totally recognition of the members of the band. There must be no ego. Every good played sound also depends of the effort from the other musicians.
Jazz interpretation involves totally recognition of the members of the band. There must be no ego. Every good played sound also depends of the effort from the other musicians.
Line 36: Line 35:
Physarum is a millions of nuclei organism, all sharing a cell wall, all operating as a single entity. They work in group...
Physarum is a millions of nuclei organism, all sharing a cell wall, all operating as a single entity. They work in group...


'''Fourth thought'''
===Fourth thought===


Jazz its influenced by early forms of blues music, wich is a form of folk music wich arose in part from the work songs of the African-American slave plantations. This songs are structured around a repetitive call-response pattern.
Jazz its influenced by early forms of blues music, wich is a form of folk music wich arose in part from the work songs of the African-American slave plantations. This songs are structured around a repetitive call-response pattern.
Line 44: Line 43:




SOME TEXTS, INSPIRATION AND REFERENCES...
==SOME TEXTS, INSPIRATION AND REFERENCES...==


''Jazz and Existencialism By MARITHE VAN DER AA ''
''Jazz and Existencialism By MARITHE VAN DER AA ''
Line 58: Line 57:




 
==FREE PROJECT: Inflexión==
'''FREE PROJECT: Inflexión'''




Line 86: Line 84:


*''Remedios Varo''_
*''Remedios Varo''_
[[File:Remedios-Varo-Creation-of-the-world-or-microcosm.jpg|400px]]Creación del mundo o microcosmos
"an avid believer in the inter-relatedness of all things and people, including the inter-weave of sound, light and image"[[http://www.theartstory.org/artist-varo-remedios.htm]]
"an avid believer in the inter-relatedness of all things and people, including the inter-weave of sound, light and image"[[http://www.theartstory.org/artist-varo-remedios.htm]]
"video" [https://www.youtube.com/watch?v=AL9qCPqcn9E]
"video" [https://www.youtube.com/watch?v=AL9qCPqcn9E]
Line 91: Line 92:


*''Hyeronimus Bosch''_
*''Hyeronimus Bosch''_
[[File:demonio-pajaro.jpg|400px]] demonio pájaro
"The master of the monstrous, the discoverer of the unconscious" Carl G. Jung
"The master of the monstrous, the discoverer of the unconscious" Carl G. Jung
[https://en.wikipedia.org/wiki/Hieronymus_Bosch]
[https://en.wikipedia.org/wiki/Hieronymus_Bosch]


Video homage of the musical group Bosco to the painter in his V Centenario. Rafael Alberti's poem "El Bosco" on the details of "The Garden of Delights" that inspired the poet.
Video homage of the musical group Bosco to the painter in his V Centenario. Rafael Alberti's poem "El Bosco" on the details of "The Garden of Delights" that inspired the poet.
[https://www.youtube.com/watch?v=9TW4o77Yy-E]
[https://www.youtube.com/watch?v=9TW4o77Yy-E]


*''Austin Osman Spare''
*''Austin Osman Spare''
[[File:austinosmanspare1.jpg|400px]]
(30 December 1886 – 15 May 1956) was an English artist and occultist who worked as both a draughtsman and a painter. Influenced by symbolism and art nouveau his art was known for its clear use of line, and its depiction of monstrous and sexual imagery.[4] In an occult capacity, he developed idiosyncratic magical techniques including automatic writing, automatic drawing and sigilization based on his theories of the relationship between the conscious and unconscious self. [https://en.wikipedia.org/wiki/Austin_Osman_Spare]
(30 December 1886 – 15 May 1956) was an English artist and occultist who worked as both a draughtsman and a painter. Influenced by symbolism and art nouveau his art was known for its clear use of line, and its depiction of monstrous and sexual imagery.[4] In an occult capacity, he developed idiosyncratic magical techniques including automatic writing, automatic drawing and sigilization based on his theories of the relationship between the conscious and unconscious self. [https://en.wikipedia.org/wiki/Austin_Osman_Spare]


==EXPERIMENT==
''See how Physarum reacts to sound''
===FIRST EXPERIMENT===
In order get a first approach with Physarum I read the Project "Sounds Synthesis with Slime Mould of Physarum Polycephalum" from Eduardo R. Miranda, Andrew Adamatzky and Jeff Jones [[https://arxiv.org/pdf/1212.1203.pdf]].
I took the following information, quote: ''"voltage values were capped in a range between -40mV to 40mV, which corresponds to a normal range of plasmodium activity"''.
My first steep was to compare the reaction of Physarum to this amount of voltage using MaxMSP and Arduino
[[File:Bildschirmfoto 2018-06-06 um 11.23.41.png|400px]]
[[File:Bildschirmfoto 2018-06-06 um 11.25.16.png|400px]]
====FIRST SETTING====
Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV).
[[File:First_Setting1.jpg|400px]]
[[File:First_Setting2.jpg|400px]]
Results: Physarum dies :( I guess it was to hot for them.
[[File:First_Setting.jpg|400px]]
[[File:First_SettingV.jpg|400px]]
====SECOND SETTING====
Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV). Time Lapse at home.
[[File:TML_Home_Second_Setting.jpg|400px]]
Time-lapse Video:
[https://vimeo.com/273654586]
{{#ev:vimeo|273654586}}
====THIRD SETTING====
Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV). Time Lapse at home.
[[File:Third_Setting.jpg|400px]]
Time-lapse Video:
[[https://vimeo.com/273661433?utm_source=email&utm_medium=vimeo-cliptranscode-201504&utm_campaign=29220]]
{{#ev:vimeo|273661433}}
===SECOND APROACH===
Influence Physarum with nutrients.
understand his behaviour and aesthetic virtues.
Inspiration from artists known of their work with process and experimentation with nature:
ALICE DUNSEATH
[https://vimeo.com/134635726]
{{#ev:vimeo|134635726}}
[http://blog.wispeo.com/featured-creative/stop-motion-time-lapse-and-live-action-alice-dunseath/]
ANDY GOLDSWORTHY
[http://www.artnet.com/artists/andy-goldsworthy/]
[https://www.youtube.com/watch?v=f7sZv4_0Fxg&frags=pl%2Cwn]
{{#ev:youtube|f7sZv4_0Fxg}}
===FOURTH EXPERIMENT===
Physarum feeds on concentration of protein and carbohydrate.
I peaked tree nutrients that are common on daily human nourishment and contain good levels of carbohydrates and/or proteins, also I used oat flakes because it has scientifically proven that Physarum response positively to this nutrient.
''White Rice:'' Proteins: 6.6 gr/100 gr. Carbohydrates: 81.6 gr/100 gr.
''Potatoe:''  Proteins: 1.7 gr/100 gr. Carbohydrates: 16 gr/100 gr.
''Oat Flakes:'' Proteins: 14 gr/100 gr. Carbohydrates: 66.5 gr/100 gr.
The aim of this experiment is to compare the behaviour of Physarum to the food medium and measuring aspects of performance.
Agar Preparation:
In order to compare reactions prepare basic Agar
100ml distilled water
2 gr Agar
Place the Agar on a multi-well container, let dry and cut each place in half.
[[File:EXP3_F3.jpg|400px]]
Agar with nutrients:
100 ml distilled water
12 gr popatoes/ rice / oat flakes
Boiled 2 minutes, filter and mixt with
2 gr Agar
[[File:EXP3_F1.jpg|400px]]
Place the Agar with nutrients carefully on each empty side of the multi-well container. Organice every column for one kind of nutrient
Place the Physarum on the middle of both Agar.
[[File:EXP3_F4.jpg|400px]]
Timelapse documentation:
[[https://www.youtube.com/watch?v=jRPBhZfIG6Q]]
{{#ev:youtube|jRPBhZfIG6Q}}
Conclutions: Physarum feels attracted mostly to potatoes
===FIFTH EXPERIMENT===
Mixture of potatoes and oat flakes
Agar Preparation:
In order to compare reactions prepare basic Agar
100ml distilled water
2 gr Agar
Place the Agar on a multi-well container, let dry and cut each place in half.
Agar with nutrients:
100 ml water
12 gr cooked potatoes
12 gt oat flakes
(mix together, filter)
2 gr Agar
Mix all together and boiled for 2 minutes
[[File:EXP4_F1.jpg|400px]]
Place the Agar with nutrients carefully on each empty side of the multi-well container.
[[File:EXP4_F2.jpg|400px]]
Timelapse documentation:
[[https://www.youtube.com/watch?v=ScYj4Bf0BoU&frags=pl%2Cwn]]
{{#ev:youtube|ScYj4Bf0BoU}}
I feed also Physarum with just this combination of nutrients and conclude that it is growing rapidly, strong and yellowish as normal.
[[File:EXP4_F3.jpg|400px]]
===SIXTH EXPERIMENT===
How Physarum reacts to a super nutrient in form of a pattern.
Agar Preparation:
In order to compare reactions prepare basic Agar
100ml distilled water
2 gr Agar
Agar with super nutrient:
100 ml from liquid taken from the mixture of Oats and raw Potatoes (24 Hours rest).
Tryptone/Peptone 0,10 gr.
Glucoce 0,15 gr.
Place the basic Agar on a Petridish, let it dry and then draw a pattern on the top with the Agar that contains a super nutrient, let dry and place Physarum
[[File:Captura de pantalla 2018-06-28 a las 17.05.07.png|400px]]
Timelapse documentation:
[https://www.youtube.com/watch?v=Gu-giqBNdWk&frags=pl%2Cwn]
{{#ev:youtube|Gu-giqBNdWk}}
[[File:Captura de pantalla 2018-06-28 a las 17.05.22.png|400px]]
[[File:Captura de pantalla 2018-06-28 a las 17.05.30.png|400px]]
[[File:Captura de pantalla 2018-06-28 a las 17.05.38.png|400px]]
===SEVENTH EXPERIMENT===
Try to make Physarum feed and grow from the Super nutrient prepare on Experiment 6.
Agar with super nutrient:
100 ml from liquid taken from the mixture of Oats and raw Potatoes (24 Hours rest).
Tryptone/Peptone 0,10 gr.
Glucoce 0,15 gr.
Physarum reaction after 2 days:
[[File:SEPT1.jpg|400px]]
[[File:SEPT4.jpg|400px]]
In the nutrient it is also growing a bacteria, generally Physarum looks frail.
[[File:SEPT3.jpg|400px]]
In most cases the bacteria occupied more space than Physarum
[[File:SEPT2.jpg|400px]]
[[File:SEPT6.jpg|400px]]
[[File:SEPT5.jpg|400px]]
[[File:SEPT7.jpg|400px]]
In a total of 14 cases, only 3 barely survived and 2 remained strong.
[[File:SEPT9.jpg|400px]]
[[File:SEPT8.jpg|400px]]
These last two were previously fed with the nutrient of experiment 5.
[[File:SEPT13.jpg|400px]]
I took one of this, feed it with oat flakes and experiment with direct light.
[[File:EXPLUZ1.jpg|400px]]
TimeLapse:
[https://www.youtube.com/watch?v=-Vync3LZP08]
{{#ev:youtube|-Vync3LZP08}}
Second TimeLapse using 9 LED lights located in line from below:
[https://youtu.be/Ur-rsnHKyc4]
{{#ev:youtube|Ur-rsnHKyc4}}
===EIGHTH EXPERIMENT===
The purpose of this experiment is to improve the resistance of Physarum. Also to trained it to feed on a proven and previously accepted nutrient.
On the FIFTH EXPERIMENT it was observed that after 5 days with  potatoe and oat nutrients Physarum was growing rapidly, strong and yellowish as normal.
Agar with nutrients from the FIFTH EXPERIMENT:
100 ml water
12 gr cooked potatoes
12 gt oat flakes
(mix together)
2 gr Agar
Mix all together and boiled for 2 minutes
I place the survivors of the last experiment on this new nutrient and observe that 9 of 12 cases are responding positiv to this environment.
[[File:SEPT10.jpg|400px]]
[[File:SEPT11.jpg|400px]]
[[File:SEPT12.jpg|400px]]
After 3 days I chose the strongest ones, they resisted the invasion of spores and created strong connections between food sources:
[[File:EXP8masfuertes.jpg|400px]]
[[File:EXP8masfuertes2.jpg|400px]]
Then I prepared Agar with nutrients from the FIFTH EXPERIMENT but this time filter it 2 times in order to obtain a transparent agar for aesthetic proposes and experiment with ligth
[[File:EXP8luzynutrientestransparentes.jpg|400px]]
I placed  Physarum on this nutrient and set a line of LEDs below the petri dish.
[[File:EXP9LuzLEDenagartransparente.jpg|400px]]
TimeLapse:
[https://youtu.be/28lOWP34Akc]
{{#ev:youtube|28lOWP34Akc}}
Conclusion:
1. At first Physarum is avoiding light and creating strong Plasmodial Veins above the light.
[[File:PyLED1.jpg|400px]]
[[File:PyLED2.jpg|400px]]
2. After 20 Hours of recording Physarum ignores light and moves into the center in search for food.
[[File:PyLED3.jpg|400px]]
[[File:PyLED4.jpg|400px]]
3. At the end of the recording (80 Hours approx.) Physarum has totally ignored the light and moves to the left in search for food. The Agar with nutrients ist almost dry.
[[File:PyLED5.jpg|400px]]
[[File:PyLED6.jpg|400px]]
== NINETH EXPERIMENT ==
Combining Agar, Agar with nutrients in form of patterns and light.
[[File:EXP9.jpg|400px]]
After 10 hours of exposure
[[File:EXP92.jpg|400px]]
After 25 hours of exposure
[https://youtu.be/g2NA03K9EKE]
{{#ev:youtube|g2NA03K9EKE}}
FINAL CONCLUTIONS:


This experiment started as a search of knowledge about the behaviour of Slime mold "Physarum Polycephalum" and its aesthetic capabilities and potential uses in the animation area. First through sound, then through the influence of light and at the end through the combination of different food sources.
It was important to replace oat flakes , wich have been used in almost every experimentation with Slime mould and in this manner develop an aesthetic that allows us to appreciate the organism structure and its ways of displacement and behaviour in different environments.


*''Leonora Carrington''
During the experimentation, the interaction of Physarum and nutrients with high protein and carbohydrates levels was more and more remarkable. Its response to these nutrients showed that there is the possibility of training the Slime mould up to three generations so that it prefers to feed on a type of nutrient and displace it self through patterns drawn with this nutrient.
[[File:Captura de pantalla 2018-05-09 a las 12.51.04.png|400px]]
Achieving this process there is the possibility of removing the organism from its purely biological context and getting it to become a pictorial tool or a performer in a micro scenario.
La reina de los mandriles
Not just its recognition and search of the food source but also this combined with the interaction with light, build suitable aesthetics to work with in the Animation and Multimedia area. This six months experimentation allows to have a base of knowledge in the potentials and different contexts of Slime Mould for further projects.

Latest revision as of 13:11, 22 May 2019

Project Ideas

  • analog animation
  • jazz music
  • experience dynamics of an organism
  • stop motion/animation


My first Physarum polycephalum

My first physarum.jpg


Some thoughts and peripheral thinking about Jazz and Physarum


First thought

Jazz its difficult to define cause contains many sub-genres and improvisation its one of its key elements. / Physarum its also for me difficult to define. Its one of 700 species of slime mold, ist a procaryote organism that in order to function reunites with millions of nuclei to maximise its means.


Second thought

Jazz is essentially a improvisation musik style. It evoques the myth of resistance. Jazz focus on individual freedom and an authentic expression of oneself, as well as the concept of living in the present. / Physarum polycephalum conceive the essence of improvisation for survival. It spreads, seeks for food and in every steep recognise it self with the intention of prevail.


Third thought

Jazz interpretation involves totally recognition of the members of the band. There must be no ego. Every good played sound also depends of the effort from the other musicians. / Physarum is a millions of nuclei organism, all sharing a cell wall, all operating as a single entity. They work in group...

Fourth thought

Jazz its influenced by early forms of blues music, wich is a form of folk music wich arose in part from the work songs of the African-American slave plantations. This songs are structured around a repetitive call-response pattern. / The particularly way that Physarum grows also respond to a repetitive call-response pattern. They go two steeps forward and one backwards, then two forwards and one backwards and so on... They talk to each order und response, then grow...


SOME TEXTS, INSPIRATION AND REFERENCES...

Jazz and Existencialism By MARITHE VAN DER AA [1]


Heather Barnett TEDTalk [2]


Jazz WIKIPEDIA [3]


FREE PROJECT: Inflexión

Direction, design and production of a short film/music video for the Jazz band "Jazz the Roots"

JazztheRoots BandCamp Song N. 11 "Inflexión" [4]


ANIMATION_esthetic and concept:

As main concept I have chosen the myth of "the awakening" and its meanings of nature, chance, revelations, natural process, ways of existing, micro decisions and existentialism.

I collect plants and botanic stuff in order to understand and take some inspiration about micro structures and botanic growth processes so I can define aesthetics and animation lines and movements.

Referencias botanicas 2.jpg

Referencias botanicas.jpg


I am approaching to the idea of creating a king of Eden garten, some kind of reality that shows symbiotic processes and developing relations between living and non living organisms, the seekness of power and the symbols of symmetry.


I have been seeing the work of artist like:

  • Remedios Varo_

Remedios-Varo-Creation-of-the-world-or-microcosm.jpgCreación del mundo o microcosmos

"an avid believer in the inter-relatedness of all things and people, including the inter-weave of sound, light and image"[[5]] "video" [6]


  • Hyeronimus Bosch_

Demonio-pajaro.jpg demonio pájaro


"The master of the monstrous, the discoverer of the unconscious" Carl G. Jung [7]

Video homage of the musical group Bosco to the painter in his V Centenario. Rafael Alberti's poem "El Bosco" on the details of "The Garden of Delights" that inspired the poet. [8]


  • Austin Osman Spare

Austinosmanspare1.jpg

(30 December 1886 – 15 May 1956) was an English artist and occultist who worked as both a draughtsman and a painter. Influenced by symbolism and art nouveau his art was known for its clear use of line, and its depiction of monstrous and sexual imagery.[4] In an occult capacity, he developed idiosyncratic magical techniques including automatic writing, automatic drawing and sigilization based on his theories of the relationship between the conscious and unconscious self. [9]

EXPERIMENT

See how Physarum reacts to sound

FIRST EXPERIMENT

In order get a first approach with Physarum I read the Project "Sounds Synthesis with Slime Mould of Physarum Polycephalum" from Eduardo R. Miranda, Andrew Adamatzky and Jeff Jones [[10]]. I took the following information, quote: "voltage values were capped in a range between -40mV to 40mV, which corresponds to a normal range of plasmodium activity".

My first steep was to compare the reaction of Physarum to this amount of voltage using MaxMSP and Arduino Bildschirmfoto 2018-06-06 um 11.23.41.png Bildschirmfoto 2018-06-06 um 11.25.16.png

FIRST SETTING

Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV).

First Setting1.jpg First Setting2.jpg


Results: Physarum dies :( I guess it was to hot for them.

First Setting.jpg First SettingV.jpg

SECOND SETTING

Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV). Time Lapse at home.

TML Home Second Setting.jpg

Time-lapse Video: [11]

THIRD SETTING

Two Petri dishes with Physarum in the middle, one of them with direct voltage (0 mV to 40mV). Time Lapse at home.

Third Setting.jpg

Time-lapse Video: [[12]]

SECOND APROACH

Influence Physarum with nutrients. understand his behaviour and aesthetic virtues.


Inspiration from artists known of their work with process and experimentation with nature:

ALICE DUNSEATH

[13]

[14]

ANDY GOLDSWORTHY

[15]

[16]

FOURTH EXPERIMENT

Physarum feeds on concentration of protein and carbohydrate. I peaked tree nutrients that are common on daily human nourishment and contain good levels of carbohydrates and/or proteins, also I used oat flakes because it has scientifically proven that Physarum response positively to this nutrient.


White Rice: Proteins: 6.6 gr/100 gr. Carbohydrates: 81.6 gr/100 gr.
Potatoe:  Proteins: 1.7 gr/100 gr. Carbohydrates: 16 gr/100 gr.
Oat Flakes: Proteins: 14 gr/100 gr. Carbohydrates: 66.5 gr/100 gr.


The aim of this experiment is to compare the behaviour of Physarum to the food medium and measuring aspects of performance.

Agar Preparation:

In order to compare reactions prepare basic Agar
100ml distilled water
2 gr Agar

Place the Agar on a multi-well container, let dry and cut each place in half.

EXP3 F3.jpg


Agar with nutrients:

100 ml distilled water
12 gr popatoes/ rice / oat flakes
Boiled 2 minutes, filter and mixt with 
2 gr Agar

EXP3 F1.jpg

Place the Agar with nutrients carefully on each empty side of the multi-well container. Organice every column for one kind of nutrient Place the Physarum on the middle of both Agar.

EXP3 F4.jpg

Timelapse documentation: [[17]]

Conclutions: Physarum feels attracted mostly to potatoes


FIFTH EXPERIMENT

Mixture of potatoes and oat flakes

Agar Preparation:

In order to compare reactions prepare basic Agar

100ml distilled water
2 gr Agar

Place the Agar on a multi-well container, let dry and cut each place in half.

Agar with nutrients:

100 ml water
12 gr cooked potatoes 
12 gt oat flakes
(mix together, filter)
2 gr Agar
Mix all together and boiled for 2 minutes


EXP4 F1.jpg

Place the Agar with nutrients carefully on each empty side of the multi-well container.

EXP4 F2.jpg

Timelapse documentation: [[18]]

I feed also Physarum with just this combination of nutrients and conclude that it is growing rapidly, strong and yellowish as normal.

EXP4 F3.jpg

SIXTH EXPERIMENT

How Physarum reacts to a super nutrient in form of a pattern.

Agar Preparation:

In order to compare reactions prepare basic Agar

100ml distilled water
2 gr Agar

Agar with super nutrient:

100 ml from liquid taken from the mixture of Oats and raw Potatoes (24 Hours rest).
Tryptone/Peptone 0,10 gr.
Glucoce 0,15 gr.

Place the basic Agar on a Petridish, let it dry and then draw a pattern on the top with the Agar that contains a super nutrient, let dry and place Physarum

Captura de pantalla 2018-06-28 a las 17.05.07.png

Timelapse documentation: [19]

Captura de pantalla 2018-06-28 a las 17.05.22.png

Captura de pantalla 2018-06-28 a las 17.05.30.png

Captura de pantalla 2018-06-28 a las 17.05.38.png


SEVENTH EXPERIMENT

Try to make Physarum feed and grow from the Super nutrient prepare on Experiment 6.

Agar with super nutrient:

100 ml from liquid taken from the mixture of Oats and raw Potatoes (24 Hours rest).
Tryptone/Peptone 0,10 gr.
Glucoce 0,15 gr.


Physarum reaction after 2 days:

SEPT1.jpg SEPT4.jpg

In the nutrient it is also growing a bacteria, generally Physarum looks frail.

SEPT3.jpg

In most cases the bacteria occupied more space than Physarum

SEPT2.jpg SEPT6.jpg SEPT5.jpg SEPT7.jpg

In a total of 14 cases, only 3 barely survived and 2 remained strong.


SEPT9.jpg SEPT8.jpg

These last two were previously fed with the nutrient of experiment 5.

SEPT13.jpg

I took one of this, feed it with oat flakes and experiment with direct light.

EXPLUZ1.jpg


TimeLapse: [20]


Second TimeLapse using 9 LED lights located in line from below: [21]


EIGHTH EXPERIMENT

The purpose of this experiment is to improve the resistance of Physarum. Also to trained it to feed on a proven and previously accepted nutrient. On the FIFTH EXPERIMENT it was observed that after 5 days with potatoe and oat nutrients Physarum was growing rapidly, strong and yellowish as normal.

Agar with nutrients from the FIFTH EXPERIMENT:

100 ml water
12 gr cooked potatoes 
12 gt oat flakes
(mix together)
2 gr Agar
Mix all together and boiled for 2 minutes

I place the survivors of the last experiment on this new nutrient and observe that 9 of 12 cases are responding positiv to this environment.

SEPT10.jpg SEPT11.jpg SEPT12.jpg


After 3 days I chose the strongest ones, they resisted the invasion of spores and created strong connections between food sources:

EXP8masfuertes.jpg EXP8masfuertes2.jpg

Then I prepared Agar with nutrients from the FIFTH EXPERIMENT but this time filter it 2 times in order to obtain a transparent agar for aesthetic proposes and experiment with ligth

EXP8luzynutrientestransparentes.jpg


I placed Physarum on this nutrient and set a line of LEDs below the petri dish.

EXP9LuzLEDenagartransparente.jpg


TimeLapse:

[22]

Conclusion:

1. At first Physarum is avoiding light and creating strong Plasmodial Veins above the light.

PyLED1.jpg PyLED2.jpg


2. After 20 Hours of recording Physarum ignores light and moves into the center in search for food.

PyLED3.jpg PyLED4.jpg

3. At the end of the recording (80 Hours approx.) Physarum has totally ignored the light and moves to the left in search for food. The Agar with nutrients ist almost dry.

PyLED5.jpg PyLED6.jpg

NINETH EXPERIMENT

Combining Agar, Agar with nutrients in form of patterns and light.


EXP9.jpg

After 10 hours of exposure


EXP92.jpg

After 25 hours of exposure

[23]


FINAL CONCLUTIONS:

This experiment started as a search of knowledge about the behaviour of Slime mold "Physarum Polycephalum" and its aesthetic capabilities and potential uses in the animation area. First through sound, then through the influence of light and at the end through the combination of different food sources. It was important to replace oat flakes , wich have been used in almost every experimentation with Slime mould and in this manner develop an aesthetic that allows us to appreciate the organism structure and its ways of displacement and behaviour in different environments.

During the experimentation, the interaction of Physarum and nutrients with high protein and carbohydrates levels was more and more remarkable. Its response to these nutrients showed that there is the possibility of training the Slime mould up to three generations so that it prefers to feed on a type of nutrient and displace it self through patterns drawn with this nutrient. Achieving this process there is the possibility of removing the organism from its purely biological context and getting it to become a pictorial tool or a performer in a micro scenario. Not just its recognition and search of the food source but also this combined with the interaction with light, build suitable aesthetics to work with in the Animation and Multimedia area. This six months experimentation allows to have a base of knowledge in the potentials and different contexts of Slime Mould for further projects.