Marie EBEL (talk | contribs) No edit summary |
Marie EBEL (talk | contribs) No edit summary |
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Step 3. Electrophoresis | Step 3. Electrophoresis | ||
#First attempt | => #First attempt | ||
[[File:STEP 3.1.JPG|400px]] | [[File:STEP 3.1.JPG|400px]] | ||
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=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | => I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). | ||
So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt | ||
=> #Second attempt | => #Second attempt | ||
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#Third attempt | |||
=> #Third attempt | |||
[[File:STEP 3.3.1.jpg|400px]] | [[File:STEP 3.3.1.jpg|400px]] | ||
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So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt | ||
#Fourth attempt | |||
=> #Fourth attempt | |||
[[File:STEP 3.4.1.jpg|400px]] | [[File:STEP 3.4.1.jpg|400px]] | ||
Revision as of 19:29, 17 January 2018
// HUMAN DNA ANALYSIS //
// PRACTICING PCR ANALYSIS //
Step 1. Extracting DNA from blood and from pork liver meat
Step 2. STR-analysis with pork meat primer
Step 3. Electrophoresis
=> #First attempt
=> I got no results : either the ladder nor the DNA samples were "growing" as they should (multiple lines). So I decided to test the ladder only to see if there is a problem from the samples themselves or with the preparation (agarose + buffer TAE 50x+ distilled water) => #Second attempt
=> #Second attempt
=> Same problem : the ladder was growing as one bloc only (see the little drawing). Even during a 2.2 attempt with new SERVA Stain G (which I thought it could be the problem, since the previous tube was quiet old). Then, I noticed that the positive side was turning in blue color and some part of the agarose gel was melting down. So I decided to test if this bleu color is coming from oxidation of the electrodes themselves, or if the ladder was "swimming" on the extra amount of solution (buffer TAE 50x + distilled water) above the agarose to reach the positive side => #Third attempt
=> #Third attempt
=> Still turning bleu. Even with smaller electrodes that are completely covered by the solution (buffer TAE 50x + distilled water) So I decided to test if this oxidation is coming from the buffer or if it is simply a natural process => #Fourth attempt
=> #Fourth attempt
=> First picture : distilled water only. Second picture : distilled water + buffer TAE 50x. No changes at all. So it might come from the SERVA Stain G (which is yellow-ish) or it is actually not a problem and does not affect on the electrophoresis itself.