GMU:BioArt WS16/Freya Probst: Difference between revisions

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Miga

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 =Final Project=
-==Bacterial Skins==
+==Skin Variations==
-:In my final project, I was interested to work with bacterial paper that is generated as a byproduct of the bacteria that lives within Kombucha, a probiotic drink. My first step was to establish an overview of what kind of micororganisms live in the Kombucha culture that Miga brought from Berlin, because cultures, from all over the planet, developed differently after they were passed on from person to person.
+:Cellulose is generated as a byproduct of the mircroorganisms that live within Kombucha, a probiotic drink. The aim of this study was to generate skins with different properties by varying the drink or the combination of the organisms.
-:Furthermore, I tried to recombine the isolated cultures of yeasts and bacteria to connect certain characteristics of the bacterial skin with certain microorganisms. I also tried to change the properties of the skins by varying the drink.
 '''1. Culture isolation of the most common microorganisms that were found in Kombucha:'''
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 |-
 | [[File:LACTOCOCCUS 1-c.jpg |250px]]
-| [[File:LACTOCOCCUS 2-c.jpg|250px]]
+| [[File:LACTOCOCCUS 2-c.jpg |250px]]
-|
+| [[File:LACTOCOCCUS 3-c.jpg |250px]]
 |-
 | [[File:LACTOCOCCUS 1-d.jpg |250px]]
@@ Line 370: / Line 368: @@
 | [[File:LACTOBACILLUS 1-a.jpg |250px]]
 | [[File:LACTOBACILLUS 2-a.jpg |250px]]
-|
+| [[File:LACTOBACILLUS 3-a.jpg |250px]]
 |-
 | [[File:LACTOBACILLUS 1-b.jpg |250px]]
 | [[File:LACTOBACILLUS 2-b.jpg |250px]]
-|
+| [[File:LACTOBACILLUS 3-b.jpg |250px]]
 |-
 | [[File:LACTOBACILLUS 1-c.jpg |250px]]
 | [[File:LACTOBACILLUS 2-c.jpg |250px]]
-|
+| [[File:LACTOBACILLUS 3-c.jpg  |250px]]
 |-
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 '''2. Recombination of Cultures:'''
+: I picked one colony from each medium and spread it on a newly cooked medium.
+{|
+|'''Sabouraud Agar'''
+|'''YM Agar'''
+|'''Thermus Medium'''
+|-
+| [[File:Sabouraud isolation.JPG |250px]]
+| [[File:YM Agar isolation.JPG |250px]]
+| [[File:Thermus isolation.jpg |250px]]
+|-
+|}
+{|
+|'''Lactococcus'''
+|'''Lactobacillus'''
+|-
+| [[File:Lactococcus isolation.JPG |250px]]
+| [[File:Lactobacillus isolation.JPG |250px]]
+|-
+|}
+I tried to recombine these bacteria in 16 probes, but after a week,  mold spread on the liquid, because the process of fermentation was missing.
+[[File:Kombucha recombination.jpeg|500px]]
+[[File:Kombucha recombination 2.jpg|500px]]
+[[File:Kombucha recombination 3.jpg|500px]]
 '''3. Kombucha-colony in various drinks:'''
+: I wondered how the choice of drink could affect the material properties of the Kombucha skin. As first reference point I looked at the ingredients of green tea and what else would contain these substances. Futhermore I did some research on where the Gluconabacter lives in nature.
 [[File:Drinks.jpg |500px]]
+I added 1ml Kombucha to 30 ml of an other drink, that could nourish the microorganisms.
+{|
+|'''corn juice'''
+|'''beer'''
+|'''carrot juice'''
+|-
+| [[File:Corn juice skin.jpg |250px]]
+| [[File:Beer skin.jpg |250px]]
+| [[File:Carrot skin.jpg |250px]]
+|-
+|}
+{|
+|'''superfruit juice'''
+|'''apple juice'''
+|'''coconut juice'''
+|-
+| [[File:Superfruit skin.jpg |250px]]
+| [[File:Apple juice skin.jpg |250px]]
+| [[File:Coconut juice skin.jpg |250px]]
+|-
+|}