GMU:BioArt WS16/Transgenic organisms: Difference between revisions

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-Transgenic organisms
 ==Related projects==
 ===mythological hybrids===
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 ===GloFish===
-https://www.google.de/search?q=GloFish&source=lnms&tbm=isch&sa=X&ved=0ahUKEwiRvcLXmafRAhUKFywKHcB3DCEQ_AUICCgB&biw=1255&bih=799
+https://www.google.de/search?q=GloFish&tbm=isch
 The first genetically modified animal to be sold as a pet
-===Revital Cohen and Tuur Van Balen===
+===Eduardo Kac===
-Sterile, http://www.cohenvanbalen.com/work/sterile
+GFP Bunny, 2000 http://www.ekac.org/gfpbunny.html#gfpbunnyanchor
-In Sterile, albino goldfish were engineered to hatch without reproductive organs. Following a long collaboration, an edition of 45 goldfish was produced for the artists by Professor Yamaha in his laboratory in Hokkaido, Japan. The fish were not conceived as animals but made as objects, unable to partake in the biological cycle. http://www.cohenvanbalen.com/work/sterile
-===Eduardo Kac===
+"My transgenic artwork "GFP Bunny" comprises the creation of a green fluorescent rabbit, the public dialogue generated by the project, and the social integration of the rabbit."
-GFP Bunny. http://www.ekac.org/gfpbunny.html
-Edunia. Source: ekac.org
+Edunia, http://www.ekac.org/nat.hist.enig.html
 Natural history of the Enigma. Edunia. "The new flower is a Petunia strain that I invented and produced through molecular biology. It is not found in nature. The Edunia has red veins on light pink petals and a gene of mine is expressed on every cell of its red veins, i.e., my gene produces a protein in the veins only [2]. The gene was isolated and sequenced from my blood. The petal pink background, against which the red veins are seen, is evocative of my own pinkish white skin tone. The result of this molecular manipulation is a bloom that creates the living image of human blood rushing through the veins of a flower." http://www.ekac.org/nat.hist.enig.html
+===Revital Cohen and Tuur Van Balen===
+Sterile, http://www.cohenvanbalen.com/work/sterile
-===Super Cell===
+In Sterile, albino goldfish were engineered to hatch without reproductive organs. Following a long collaboration, an edition of 45 goldfish was produced for the artists by Professor Yamaha in his laboratory in Hokkaido, Japan. The fish were not conceived as animals but made as objects, unable to partake in the biological cycle. http://www.cohenvanbalen.com/work/sterile
-The project Super Cell is a fictional supermarket offering speculative products, which are all based on Synthetic Biology. The projects aims to improve public awareness about Synthetic Biology and at the same time, foster a debate on how we want to see it manifested in our everyday lives. Implications of Biotechnologies are usually discussed on a philosophical, ethical, legal or possibly religious level. https://vimeo.com/39653216 http://super-cell.org/
 ===Joe Davis===
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 "Among the most important tools in the genetic engineer's tool kit are enzymes that perform specific functions on DNA. .. enzymes known as ligases join the ends of two DNA fragments. These and other enzymes enable the manipulation and amplification of DNA, essential components in joining the DNA of two unrelated organisms."(Fenwick 2004)
-===Methods for genetically modified plants===
+===Methods for genetically modified organisms===
 . man mischt vereinzelte Zellen mit DNA Molekülen / eg using heat stress
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 Needed:
-* (Reptilian) incubator
+* (Reptile) incubator
 * Spectrometer to calculate cell growth mass
-TSS medium reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the eis and then heat shock with 42 degrees warmed up water.
+TSS medium (Transformation & Storage Solution) reduce cell liquids/plazma (oils, acids, etc). Cool down e.coli cells 4 degrees (above freezing temperature). The right amount of e.coli need to be now annoyed with tss solution. We put into small tubes (each 1 ml) e.coli with removed previous LB medium/solution (centrifuge for 10 min 3000 rpm) and we add tss solution and 5 microliter dna. 8 positive and two negative, with no dna. We keep 30 min in the ice and then heat shock with 42 degrees warmed up water.
 Day 2
 Results are good. With Arabinose sugars we will switch the GFP gene on (so far GFP is not active). Arabinose activates promoter (the small part of the gene) plasmid (a small part of chromosome). Filter new LB-AMP-ARA medium. Pick one colony of bacteria and put together with a tip into a new LB-Amp-Ara medium. Put into incubator shaker for a night
+Day 3
+https://www.google.de/search?q=gfp+e+coli&tbm=isch
 == References ==
 * wikipedia (2015a). Transgenesis, https://en.wikipedia.org/wiki/Transgenesis
 * wikipedia (2015b). Shapeshifting, https://en.wikipedia.org/wiki/Shapeshifting