GMU:DIY Biolab “Driver’s License”/Designing primers, PCR: Difference between revisions
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Dr. Julian Chollet will perform a STR-analysis on a single region/loci of your genome - with the DNA that you extracted the week before. Usually, 12-15 different ‘loci’ are analyzed to get a reliable result, which means that our experiment will not reconstruct potential family relationships within the group. But if two participants are closely related, it is highly probable that they will show the same fragment size. The workshop is about learning basic molecular biology techniques and understanding the concepts as well as the chemistry behind DNA. | Dr. Julian Chollet (DE) will perform a STR-analysis on a single region/loci of your genome - with the DNA that you extracted the week before. Usually, 12-15 different ‘loci’ are analyzed to get a reliable result, which means that our experiment will not reconstruct potential family relationships within the group. But if two participants are closely related, it is highly probable that they will show the same fragment size. The workshop is about learning basic molecular biology techniques and understanding the concepts as well as the chemistry behind DNA. | ||
STR-ANALYSIS - modified from https://en.wikipedia.org/wiki/STR_analysis | STR-ANALYSIS - modified from https://en.wikipedia.org/wiki/STR_analysis | ||
Revision as of 10:46, 8 November 2017
Dr. Julian Chollet (DE) will perform a STR-analysis on a single region/loci of your genome - with the DNA that you extracted the week before. Usually, 12-15 different ‘loci’ are analyzed to get a reliable result, which means that our experiment will not reconstruct potential family relationships within the group. But if two participants are closely related, it is highly probable that they will show the same fragment size. The workshop is about learning basic molecular biology techniques and understanding the concepts as well as the chemistry behind DNA.
STR-ANALYSIS - modified from https://en.wikipedia.org/wiki/STR_analysis
A Short Tandem Repeat (STR) analysis is one of the most useful methods in molecular biology which is used to compare specific loci on DNA from two or more samples. A short tandem repeat is a microsatellite, consisting of a unit of two to thirteen nucleotides repeated hundreds of times in a row on the DNA strand. STR analysis measures the exact number of repeating units. The probes are attached to desired regions on the DNA, and a polymerase chain reaction (PCR) is employed to discover the lengths of the short tandem repeats. STR-analysis is mainly used for forensic analysis and proof of kinship, e.g. a paternity test.