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→Workshop 1 (Molecular/DNA cloning)
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*4. divide 200 ul of mix into tubes with samples so it dissolves all other proteins and leaves only dna | *4. divide 200 ul of mix into tubes with samples so it dissolves all other proteins and leaves only dna | ||
*5. putting samples into heating/cooling block for one hour with 56C | *5. putting samples into heating/cooling block for one hour with 56C | ||
*6. transfer liquid to the bigger epic tubes | *6. transfer liquid to the bigger epic tubes | ||
*7. adding 200 ul of lüser puffer to the dissolved samples. brakes the walls of the cell (functions as washing medium) | *7. adding 200 ul of lüser puffer to the dissolved samples. brakes the walls of the cell (functions as washing medium) | ||