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== Quick and dirty Euglena tracking using OpenCV, Syphon, and OSC. == | === Quick and dirty Euglena tracking using OpenCV, Syphon, and OSC. === | ||
Sources at GitHub: https://github.com/lscherff/biogames/tree/master/TrackEuglenaSyphonOSC | |||
=== Threshold based Euglena detection === | === Threshold based Euglena detection === | ||
<source lang="java"> | <source lang="java"> | ||
opencv.loadImage(cam); | opencv.loadImage(cam); | ||
| Line 10: | Line 10: | ||
opencv.gray(); | opencv.gray(); | ||
opencv.invert(); | opencv.invert(); | ||
opencv.erode(); | opencv.erode(); | ||
opencv.dilate(); | opencv.dilate(); | ||
threshold = int(map(mouseX,0,width,0,255)); | |||
opencv.threshold(threshold); | opencv.threshold(threshold); | ||
| Line 22: | Line 22: | ||
</source> | </source> | ||
=== Skipping large/small contours === | === Skipping large/small/malformed contours === | ||
<source lang="java"> | <source lang="java"> | ||
for (Contour contour : contours) { | for (Contour contour : contours) { | ||
| Line 30: | Line 30: | ||
if (a > minArea && wh > 0.1 && wh < 10 && a < maxArea) { | if (a > minArea && wh > 0.1 && wh < 10 && a < maxArea) { | ||
// ... | // ... | ||
</source> | |||
=== Maintaining Euglena identity in a list (based on proximity from frame to frame), computing speed as dx, dy and mean speed as mdx, mdy=== | |||
<source lang="java"> | |||
void checkEuglena(Contour c) { | |||
boolean found = false; | |||
Rectangle r = c.getBoundingBox(); | |||
for (Euglena e : euglenaList) { | |||
if (dist(e.x, e.y, r.x, r.y) < distanceThreshold) { | |||
e.dx = e.x - r.x; | |||
e.dy = e.y - r.y; | |||
e.x = r.x; | |||
e.y = r.y; | |||
e.found = true; | |||
found = true; | |||
break; | |||
} | |||
} | |||
if (!found) { | |||
euglenaList.add(new Euglena(r.x, r.y)); | |||
} | |||
} | |||
void deleteEuglena() { | |||
ListIterator<Euglena> iter = euglenaList.listIterator(); | |||
float newmdx = 0; | |||
float newmdy = 0; | |||
while (iter.hasNext()) { | |||
Euglena e = iter.next(); | |||
if (e.found==false) iter.remove(); | |||
else { | |||
e.found = false; | |||
newmdx = newmdx + e.dx; | |||
newmdy = newmdy + e.dy; | |||
} | |||
} | |||
if (euglenaList.size() > 0) { | |||
newmdx = newmdx / euglenaList.size(); | |||
newmdy = newmdy / euglenaList.size(); | |||
} else { | |||
newmdx = 0; | |||
newmdy = 0; | |||
} | |||
mdx += (newmdx - mdx)*0.01; | |||
mdy += (newmdy - mdy)*0.01; | |||
} | |||
</source> | </source> | ||