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''Dead Life Live Life'' | '''Dead Life Live Life''' is a poetic exploration of the interconnectedness of life and death through the sonification of the DNA movement.� It sonifies the DNA movement of a persimmon tree planted at my grandmother's house, who passed away in 2018. The tree serves as a living memory of her, still enduring in the now-empty house. The project visualizes the movement of the tree’s DNA through electrophoresis experiments, converting this movement into sound in real time. | ||
[[File:Diagram Dead LifeLiveLife.jpg|center|thumb|800x800px]] | [[File:Diagram Dead LifeLiveLife.jpg|center|thumb|800x800px]] | ||
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</gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber | </gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber | ||
* �Extra Guide of Electrophoresis https://blog.naver.com/kmkae52/222804356219<br /> | |||
=====2. Sound part===== | =====2. Sound part===== | ||
1) Sonification of DNA movementMax/Msp patch | 1) Sonification of DNA movementMax/Msp patch | ||
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2) Sound Design | 2) Sound Design | ||
===== *reference : Paul '''''Latent Figure Protocol'', Paul Vanouse, 2007-09''' https://www.paulvanouse.com/lfp.html ===== | |||
====Work in Biolab==== | ====Work in Biolab==== | ||
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I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives. | I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives. | ||
===== '''2024.12.11''' ===== | |||
'''2024.12.11''' | |||
[[File:Pcr 29241211.jpg|thumb]] | [[File:Pcr 29241211.jpg|thumb]] | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry. | I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry. | ||
<u>Result</u> | |||
During the PCR experiment, I successfully amplified all the sample DNA and proceeded with gel electrophoresis. However, I encountered an error message indicating that no electricity was flowing through the gel, which prevented me from continuing the experiment. | |||
===== '''2024.12.17. Tue''' ===== | |||
<u>Introduction</u> | |||
Electrophoresis was performed under constant UV light and the movement of the dna was filmed live. Experiments with DNA Ladder. | |||
<u>Result</u> | |||
<nowiki>https://youtu.be/OsDsI0Ecbg4?si=UxQGSdfn7OdSeWgl</nowiki> | |||
Under constant UV light, the DNA becomes increasingly blurry. I feel that it is not against the artistic message of this work that the DNA gradually disappears as an inherent property during the experiment. |
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