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GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
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''Dead Life Live Life'' | {{#ev:youtube|bGzmhJ9kQi4}} | ||
=== '''Dead Life Live Life (working title)''' === | |||
'''Dead Life Live Life''' is a poetic exploration of the interconnectedness of life and death through the sonification of the DNA movement.� It sonifies the DNA movement of a persimmon tree planted at my grandmother's house, who passed away in 2018. The tree serves as a living memory of her, still enduring in the now-empty house. The project visualizes the movement of the tree’s DNA through electrophoresis experiments, converting this movement into sound in real time. | |||
[[File:Diagram Dead LifeLiveLife.jpg|center|thumb|800x800px]] | |||
[[File:Dlll 1.001.jpg|center|frameless|800x800px]] | [[File:Dlll 1.001.jpg|center|frameless|800x800px]] | ||
[[File:Dlll 2..jpg|center|frameless|800x800px]] | [[File:Dlll 2..jpg|center|frameless|800x800px]] | ||
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==== Technical solution ==== | ====Technical solution==== | ||
===== 1. Biological part ===== | =====1. Biological part===== | ||
Instruction of DNA analysis<gallery> | Instruction of DNA analysis<gallery> | ||
File:Instruction1 DNAworkshop.jpg | File:Instruction1 DNAworkshop.jpg | ||
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</gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber | </gallery>DNA of the persimmon is amplified through a PCR experiment. The amplified DNA is visualized in a gel electrophoresis experiment. DNA molecules dyed to respond to UV light glow and flow electromagnetically in an agar-gel chamber | ||
* �Extra Guide of Electrophoresis https://blog.naver.com/kmkae52/222804356219<br /> | |||
=====2. Sound part===== | |||
===== 2. Sound part ===== | |||
1) Sonification of DNA movementMax/Msp patch | 1) Sonification of DNA movementMax/Msp patch | ||
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2) Sound Design | 2) Sound Design | ||
==== | ===== *reference : Paul '''''Latent Figure Protocol'', Paul Vanouse, 2007-09''' https://www.paulvanouse.com/lfp.html ===== | ||
===== '''2024.11.16. Sat''' ===== | ====Work in Biolab==== | ||
====='''2024.11.16. Sat'''===== | |||
<u>Introduction</u> | <u>Introduction</u> | ||
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<gallery>File:After 50hours gelElectrophoresis.jpg</gallery> | <gallery>File:After 50hours gelElectrophoresis.jpg</gallery> | ||
===== ''' 2024.11.18. Mon''' ===== | =====''' 2024.11.18. Mon'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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<gallery> File:DNALadder UV lighting.jpg </gallery> | <gallery> File:DNALadder UV lighting.jpg </gallery> | ||
===== '''2024.11.19. Tue''' ===== | ====='''2024.11.19. Tue'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
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</gallery> | </gallery> | ||
===== '''2024.11.20. Wed''' ===== | ====='''2024.11.20. Wed'''===== | ||
<u>Introduction</u> | <u>Introduction</u> | ||
DNA Extraction Exercise_ Kaki | DNA Extraction Exercise_ Kaki, Leaf | ||
- I failed to extract Kaki's DNA in my experiment yesterday. This time i followed | - I failed to extract Kaki's DNA in my experiment yesterday. This time i followed the guide from the result of research by Alessandro to extract Kaki's DNA. | ||
- Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside. | - Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside. | ||
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-The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf. | -The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf. | ||
<small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small> | <small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small> | ||
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<u>Result</u> | <u>Result</u> | ||
Both the leaves and the persimmon failed to extract DNA. I was a little frustrated. | Both the leaves and the persimmon failed to extract DNA. I was a little frustrated. | ||
It seems like I need to extract DNA from the leaves persimmon tree. | |||
But where do I get the leaves?<gallery> | But where do I get the leaves?<gallery> | ||
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</gallery> | </gallery> | ||
====='''2024.11.21. Thu'''===== | |||
<u>Introduction</u> | <u>Introduction</u> | ||
Various sample preparations for pcr and gel electrophoresis : DNA extraction of banana and blueberry. | Various sample preparations for pcr and gel electrophoresis : DNA extraction of banana and blueberry. | ||
<u>Procedure</u> | |||
I followed the guide from Julian's workshop as usual<gallery> | |||
File:DNA Extraction Banana-blueberry.jpg | |||
File:DNA Extraction Banana-blueberry1.jpg | |||
File:DNA Extraction Banana-blueberry2.jpg | |||
</gallery><u>Result</u> | |||
DNA extraction was successful for both bananas and blueberries. DNA extraction is particularly easy for bananas. Blueberries, like strawberries and raspberries, do not have a high DNA extraction rate compared to other berry types.<gallery> | |||
File:DNA from BANANA.jpg | |||
File:DNA from Blueberry.jpg | |||
File:DNA from blueberry in tube.jpg | |||
File:DNA from Banana in tube.jpg | |||
</gallery> | |||
====='''2024.11.23. Sat'''===== | |||
<u>Introduction</u> | |||
Exercise for DNA centrifugation and PCR Experiment | |||
<u>Procedure</u> | |||
I followed the instruction in Julian's workshop for setting up the centrifugation of DNA sample and PCR. | |||
#Centrifuge setting: 8kg, 60 seconds | |||
#PCR: see instructional photo above. | |||
A total of five DNA samples were subjected to DNA amplification by PCR | |||
*Label information | |||
*#B11: DNA of Blueberries (from 19.Nov) | |||
*#B12: DNA of Blueberries (from 19.Nov) | |||
*#B2: DNA of Blueberries (from 21.Nov) | |||
*#BA: DNA of Banana (from 21.Nov) | |||
*# K: Kaki pulp with soap, salt, hot water (from 20.Nov) | |||
<small>*sample B11, B12 are dirtier than B2</small> | |||
<small>*So far no success to extract Kaki DNA. I used this time kaki pulp instead of DNA. It seems like there are no DNA in pulp, but at least I wanted experiment with pulp until Gel-electrophoresis just for double checking. </small> | |||
<gallery> | |||
File:Centrifugation1.jpg | |||
File:Material for pcr.jpg | |||
File:Pcr1.jpg | |||
File:Pcr2.jpg | |||
File:Pcr setting bento machine.jpg | |||
<nowiki></gallery> | |||
====='''2024.11.26. Tue'''===== | |||
<u>Introduction</u> | |||
Extracting DNA from leaves using DNeasy Kit with supervising by Alessandro Volpato_ Since there are no leaves from persimmon trees, I experimented with leaves from other trees in Ilm park. | |||
<u>Procedure</u> | <u>Procedure</u> | ||
I followed the | The protocol below was followed. | ||
[[File:DNeasy protocol.pdf|left|thumb]] | |||
<nowiki>https://youtu.be/c1WOBy5vSg8?si=ksFltpYkG1ytvugT</nowiki> | |||
<gallery> | |||
File:Dneasy kit1.jpg | |||
File:Dneasy kit.jpg | |||
File:Dneasy kit2.jpg | |||
File:Dneasy kit3.jpg | |||
File:Dneasy kit4.jpg | |||
File:Dneasy kit5.jpg | |||
File:Dneasy kit6.jpg | |||
</gallery> | |||
<u>Result</u> | |||
I followed the protocol to extract DNA from the leaves, but I could not visually check if the DNA was extracted correctly. After the gel electrophoresis, i I will know if the DNA was extracted. �I will start electrophoresis as soon as the Stein green arrives. | |||
===== '''2024.12.11''' ===== | |||
[[File:Pcr 29241211.jpg|thumb]] | |||
<u>Introduction</u> | |||
PCR / Gel electrophoresis | |||
I did PCR 3 samples which are DNA of leaves extracted by DNA EXTRACT KIT, Banana, Blueberry. | |||
<u>Result</u> | <u>Result</u> | ||
During the PCR experiment, I successfully amplified all the sample DNA and proceeded with gel electrophoresis. However, I encountered an error message indicating that no electricity was flowing through the gel, which prevented me from continuing the experiment. | |||
===== '''2024.12.17. Tue''' ===== | |||
<u>Introduction</u> | |||
Electrophoresis was performed under constant UV light and the movement of the dna was filmed live. Experiments with DNA Ladder. | |||
<u>Result</u> | |||
<nowiki>https://youtu.be/OsDsI0Ecbg4?si=UxQGSdfn7OdSeWgl</nowiki> | |||
Under constant UV light, the DNA becomes increasingly blurry. I feel that it is not against the artistic message of this work that the DNA gradually disappears as an inherent property during the experiment. | |||