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GMU:Life in an aquatic ecosystem/Dahye Seo (view source)
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'''2024.11.20. Wed''' | |||
<u>Introduction</u> | |||
DNA Extraction Exercise_ Kaki (ChatGPT), Leaf | |||
- I failed to extract Kaki's DNA in my experiment yesterday. This time i followed Chat GPT's guide to extract Kaki's DNA. | |||
- Alessandro's research results show that DNA is mainly extracted from the leaves of the persimmon. Since I can't get the leaves of the persimmon right now, I tried the experiment with the weeds on the roadside. | |||
<u>Procedure</u> | |||
-The DNA extraction experiment was performed following the guide of chat gpt for the persimmon and the existing instructions for the leaf. | |||
<nowiki>*</nowiki>Chat GPT's guide | |||
<small>To extract DNA from kaki (persimmon) fruit, you'll need about '''3-4 fruits''' to get a sufficient amount of DNA for analysis. Here's a step-by-step procedure:</small> | |||
<small>### Materials Needed:</small> | |||
<small>- 3-4 kaki fruits</small> | |||
<small>- 5g washing up liquid or hand soap</small> | |||
<small>- 2g salt</small> | |||
<small>- 100ml tap water</small> | |||
<small>- 100ml ice-cold alcohol (isopropyl alcohol or ethanol)</small> | |||
<small>- Knife and fork for cutting and mashing</small> | |||
<small>- Beaker or large bowl</small> | |||
<small>- Coffee filter or fine sieve</small> | |||
<small>- Saucepan for hot water bath</small> | |||
<small>- Ice water bath</small> | |||
<small>### Procedure:</small> | |||
<small>1. '''Preparation''': Peel the kaki fruits and cut them into small chunks. Remove any seeds.</small> | |||
<small>2. '''Mashing''': Mash the fruit chunks thoroughly in a beaker or large bowl to break down the cells.</small> | |||
<small>3. '''Extraction Buffer''': Mix the washing up liquid, salt, and tap water in a separate container. Stir gently to avoid creating bubbles.</small> | |||
<small>4. '''Mixing''': Add the extraction buffer to the mashed fruit and mix well.</small> | |||
<small>5. '''Incubation''': Place the mixture in a hot water bath at '''60°C''' for '''15 minutes'''. Stir occasionally to ensure even heating.</small> | |||
<small>6. '''Cooling''': Transfer the mixture to an ice water bath and let it cool for '''5 minutes'''.</small> | |||
<small>7. '''Filtration''': Filter the mixture through a coffee filter or fine sieve into a clean container. This will remove solid debris.</small> | |||
<small>8. '''DNA Precipitation''': Slowly pour ice-cold alcohol down the side of the container to form a layer on top of the filtered mixture. DNA will precipitate out as a white, jelly-like substance at the interface.</small> | |||
<small>9. '''Collection''': Use a medicine dropper or a hook made from thin wire to collect the DNA precipitate.</small> | |||
[[File:Leaaaaf.jpg|thumb]] | |||
<u>Result</u> | |||
Both the leaves and the persimmon failed to extract DNA. I was a little frustrated. | |||
The guide for chat GPT seems unreliable. It seems like I need to extract DNA from the leaves persimmon tree. | |||
[[File:Leaaaaf1.jpg|thumb]] | |||
[[File:Dh1.jpg|thumb]] | |||
[[File:Dh2.jpg|thumb]] | |||
[[File:Dh3.jpg|thumb]] | |||
[[File:Dh4.jpg|thumb]] | |||
[[File:Dh5.jpg|thumb]] | |||
[[File:Dh6.jpg|thumb]] | |||
[[File:Dh7.jpg|thumb]] | |||
[[File:Dh8.jpg|thumb]] | |||
But where do I get the leaves? | |||