GMU:BioArt Forum/Endophyte Club: Difference between revisions

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Miga

Bureaucrats, emailconfirmed, Administrators

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+<gallery>
+File:Screen Shot 2022-11-28 at 1.33.51 PM.png
+File:Screen Shot 2022-11-28 at 1.33.56 PM.png
+</gallery>
 ==Endophyte Club==
 At the Endophyte Club biological hobbyists meet to identify, discover, discuss and trade information about new, rare and hard to access microorganisms. The race is on: who will get to unlock, collect and share the secrets of the microbiome era?
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 ==Workshop Overview==
-We would propose running a 2 day lab where participants learn how to collect agricultural plant samples (leaf, roots, etc.) and plate, grow, isolate and identify the microorganisms that grow inside the plants (Endophytes). The goal of the lab would be to discover an entirely new organism and to take this DIY Bio research forward and to prototype its promise and limitations.
+During a 2 day lab participants learn how to collect agricultural plant samples (leaf, roots, etc.) and plate, grow, isolate and identify the microorganisms that grow inside the plants (Endophytes). The goal of the lab is to discover an entirely new organism and to take this DIY Bio research forward and to prototype its promise and limitations.
 Applied endophyte research is being promoted as a possible replacement for chemical fertilizers and pesticides. In addition to learning basic biological lab techniques, we will be able to imagine different food futures and various registers and modes of biohacking.
 http://www.endophyte.club/
 ==Time table==
-===FRIDAY (10:00-16:00)===
+===FRIDAY, 2 December 2022 (10:00-16:00)===
 *10:00 Canteen
 *16:00 Visit a supermarket
-===SATURDAY (10:00-16:00)===
+===SATURDAY, 3 December 2022 (10:00-16:00)===
 *10:00 Ilm park (tbc)
 *16:00 Lunch
+==Students==
+* [[/Antje Danz & Martin Melcher]]
+==Literature==
+*Frank M. Dugan. (2017). Identification of Fungi.
+*Jean Williams-Woodward, Simplified Fungi Identification Key, https://plantpath.caes.uga.edu/content/dam/caes-subsite/plant-pathology/extension-pdfs/PDL-fungi-key.pdf
+*https://mycokeys.pensoft.net/article/23670/
+*https://www.frontiersin.org/articles/10.3389/fmicb.2018.01707/full
+*https://www.frontiersin.org/articles/10.3389/fbioe.2021.650351/full
+*https://direct.mit.edu/books/oa-monograph/5330/chapter-standard/3809801/SymbioticA-Culturing-Art-and-Science
+*http://critical-art.net/molecular-invasion-2002/
+*https://monoskop.org/images/3/3a/Critical_Art_Ensemble_Digital_Resistance_Explorations_in_Tactical_Media.pdf
+*https://mitpress.mit.edu/9780262514910/tactical-biopolitics/
+==Identification of endophytes==
+*Isolate and continue growing on a new plate
+*Send it to sequence
+*Repeat the experiment with the same plant a couple of times
+*Isolate from different places the grown organism
+*Do microscopy
+==Results==
+===06 Dec 2022 -- miga===
+<gallery>
+File:M_IMG_1705.jpg
+File:M_IMG_1706.jpg
+File:M_IMG_1707.jpg
+File:M_IMG_1708.jpg
+File:M_IMG_1709.jpg
+File:M_IMG_1710.jpg
+</gallery>
+===15 Dec 2022 -- Klaus===
+. 2. und 3. Plating of surface sterilized Cranberry/Raspberry/Blueberry Samples
+<gallery>
+File:K_IMG_3355.jpeg
+File:K_IMG_3350.jpeg
+File:K_IMG_3349.jpeg
+</gallery>
+. 5. 6. 7. Close ups:
+„Japanische Aubergine“, produced in Spain, surface sterilized by 4 % Hypochlorid, Samples from inner fruit tissue and of surface/scin:
+<gallery>
+File:K_IMG_3332.jpeg
+File:K_IMG_3312.jpeg
+File:K_IMG_3322.jpeg
+File:K_IMG_3329.jpeg
+File:K_IMG_3340.jpeg
+File:K_IMG_3341.jpeg
+File:K_IMG_3342.jpeg
+File:K_IMG_3343.jpeg
+</gallery>
+===12 Dec 2022 -- Cosmo Schüppel===
+<gallery>
+File:P1070043.jpeg
+File:P1070044.jpeg
+File:P1070045.jpeg
+File:P1070046.jpeg
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+File:P1070059.jpeg
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+File:P1070065.jpeg
+File:P1070066.jpeg
+File:P1070067.jpeg
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+File:P1070070.jpeg
+File:P1070071.jpeg
+File:P1070072.jpeg
+File:P1070073.jpeg
+File:P1070074.jpeg
+File:P1070075.jpeg
+File:P1070076.jpeg
+</gallery>