Paola Stephania/LINK PROJECT: Difference between revisions

'''EXPERIMENTATION with Alii Vibrio Fischeri'''

* Day 1: The extraction of ''Alii Vibrio Fischeri'' from a living natural environment ''(took from an octopus)''.

* Day 4: I failed to extract the ''Alii Vibrio Fischeri'' into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made. My hypothesis is that the specimen wasn't in the same original conditions from where it comes from, and it couldn´t survive.  

CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of ''Polycephalum/physarum'' in order to confirm if this specimen can be cultivated in salty environments. According to the previous investigations of Dussutour, it is possible, so I will explore this idea.

 

* Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of ''Polycephalum/physarum'' are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl ''(simulating the salinity of the sea)'', 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium ''(the salty medium simulating the salinity of the sea)''.

 

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