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*2 Sample 0,30g malz extract, 100ml water & 0,7g NACl.
 
*2 Sample 0,30g malz extract, 100ml water & 0,7g NACl.

Revision as of 21:44, 1 December 2018

CULTIVATION OF Haematococcus Pluvialis.

- Known by its red color, it's an alga that in stress conditions produces a high content of a strong antioxidant known as astaxanthin.

* SCIENTIFIC CLASIFICATION Division: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales Family: Haematococcaceae Genus: Haematococcus Species: H. pluvialis Binomial name Haematococcus pluvialis

https://en.wikipedia.org/wiki/Haematococcus_pluvialis

HCP 1.PNG

Sequence of growth states of the Haematococcus: https://comunicarciencia.bsm.upf.edu/?tag=haematococcus-pluvialis


  • MEDIUM

- 100ml of water

- 3% of vinasse

- 0.7% of NaCl.

The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture.

Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium Malz extract. (brennwert: 1.216kJ, fett 0.2g, kohlenhydrate 65g, Zucker 45g, Eiweiss 5g, Salz0.02g.)

  • Day 1: Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7. Preparation and from left to right; original medium, 10% of original medium diluted in 100ml of water, 1% of original sample diluted in 100ml of water.

Wd7.jpg Wd8.jpg

The three samples contain:

1 Sample 3,00g malz extract, 100ml water & 0,7g NACl. 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl. 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.

  • Day 2: An observation under the microscope is made in order to see the specimen.

Hapl0.jpg

Hapl0b.jpg

  • Day 4: After 4 days of growth the specimens haven't acquired an orange color (this color was expected if the conditions of the environment were ideal for the specimen.

Wd9.JPG

  • Day 10: The specimen seems to be starting to attach to the bottom of the tube. I made an observation of the three samples under the microscope, here are the results:


Wd10.JPG

Hapl1.png

Hapl0c.jpg

From left to right more concentration of vinasse and less according to the made samples.

  • 1 Sample 3,00g malz extract, 100ml water & 0,7g NACl.

Hapl2.jpg

Hapl10.jpg

  • 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl.

Hapl5.jpg

Hapl6.jpg

Hapl7.jpg

  • 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.

Hapl8.jpg

Hapl9.jpg


Conclusions: the environmental conditions for the Haematococcus-pluvialis are not ideal, in order for it to get out of it hibernation state. My first hypothesis is that it doesn't have the ideal conditions in terms of light and temperature, and my second hypothesis is that it doesn't have the ideal medium (a vinasse medium wasn't used for the growth of the specimen, instead a similar medium was made).