Paola Stephania/LINK TO WORKSHOP: Difference between revisions

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- Whit the ''Potatoe Dextrose Agar (PDA)'' made on the first day we cultivate/make a clone of ''Armillaria Gallica''.
- Whit the ''Potatoe Dextrose Agar (PDA)'' made on the first day we cultivate/make a clone of ''Armillaria Gallica''.


[[File:wd5.jpg|400px]]    [[File:wd6.jpg|400px]]
[[File:wd5.jpg|400px]]     
 
[[File:wd6.jpg|400px]]


- A selected specimen ''Haematococcus Pluvialis'' is cultivated. The medium is made with 100ml of water, 3% of vinasse and 0.7% of NaCl. Note; in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium. Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH ''7''.
- A selected specimen ''Haematococcus Pluvialis'' is cultivated. The medium is made with 100ml of water, 3% of vinasse and 0.7% of NaCl. Note; in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium. Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH ''7''.
[[File:wd7.jpg|400px]]    [[File:wd8.jpg|400px]]
[[File:wd7.jpg|400px]]    [[File:wd8.jpg|400px]]


- A sample of Aliivibrio Fischeri is taken from a specimen brought by a member of the group. The medium is made with 3g of NaCl, 0.1g of glycerol, 1g of Pepton, 3g og mear extract in 100ml of distilled water.
- A sample of Aliivibrio Fischeri is taken from a specimen brought by a member of the group. The medium is made with 3g of NaCl, 0.1g of glycerol, 1g of Pepton, 3g og mear extract in 100ml of distilled water.

Revision as of 23:10, 10 November 2018

ACTIVITIES

During the three first days, a series of activities were made;


*Day 1:

  • Introduction to the laboratory working space, the location of instruments and some working techniques.
  • Workshop around activities, expectations, questions around what the group wants to learn, personal experience, previous work or field of work, and knowledge that can be offered to the team.
  • Presentation from Jan, an expert in fungi work. After the presentation, a medium for fungi was prepared, a medium for one of his original samples.

Original sample of Jan

Wd3.jpg Wd4.jpg

Potatoe Dextrose Agar (PDA)

- Diced and boiled potatoes - 150g - Dextrose - 7g - In 500ml of water, boil 150g of potatoes. Strain out the solids, bring the solution back to 500ml with water, then add remaining ingredients. Five grams of potatoes flakes can be used as a substitute.

For the preparation were used: - 380 ml of diced and boiled Potatoes - 7 g of Dextrose - 7.6 g of Agar

Wd1.jpg

  • Alimentation and photography exercise with Hydra Vulgaris - Graver Süsswasserpolys.

Wd2.jpg


*Day 2:

- Whit the Potatoe Dextrose Agar (PDA) made on the first day we cultivate/make a clone of Armillaria Gallica.

Wd5.jpg

Wd6.jpg

- A selected specimen Haematococcus Pluvialis is cultivated. The medium is made with 100ml of water, 3% of vinasse and 0.7% of NaCl. Note; in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium. Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7.

Wd7.jpg Wd8.jpg

- A sample of Aliivibrio Fischeri is taken from a specimen brought by a member of the group. The medium is made with 3g of NaCl, 0.1g of glycerol, 1g of Pepton, 3g og mear extract in 100ml of distilled water.