INVESTIGATION AROUND MICROORGANISM AND PHOTOGRAPHY TECHNIQUES
In order to decide which microorganism are suitable for the work that is going to be developed, an initial investigation is made in order to understand the characteristics of each microorganism, their needs in order to grow, and important information suitable for the project.
- List of bacterias and microorganism that can be cultivated in laboratories of high /medium security.
- Bacillus Subtilis - (brown)
- Chromobacterium violaceum - (violet)
- Escherichia Coli - (colourless)
- Micrococcus Luteus - (Yellow)
- Micrococcus Roseus - (Rosa)
- Proteus Mirabilis / Pseudomonas aeruginosa - (brown)
- Pseudomonas Fluorescens – (Blue or green) fluorescens with pyoverdine
- Serratia Marcescens – (Pink or Orange)
- Staphylococcus aureus – (Yellow)
- Vibrio Fischeri – (Bioluminescent)
- List of bacterias and microorganism that can be cultivated in laboratories of medium/basic security.
- Polycephalum/physarum - (Yellow) tree form https://en.wikipedia.org/wiki/Physarum_polycephalum
- Clorococcum - (green) https://en.wikipedia.org/wiki/Chlorococcum
- Euglena gracilis - (green) https://en.wikipedia.org/wiki/Euglena_gracilis https://www.youtube.com/watch?v=UWSBcaJE6x4
- Euglena Spirogyra / Lepocinclis Spirogyroides - (green/yellow)
- Haematococcus Pluvialis - (red) https://en.wikipedia.org/wiki/Haematococcus_pluvialis https://www.youtube.com/watch?v=c6cCpyivi9E
- Volvox Aureus - (granulated green) https://en.wikipedia.org/wiki/Volvox https://www.youtube.com/watch?v=lLvmHaz1uIw
- Sternalge / Zygnema spec - (yellow/green) remoline form https://en.wikipedia.org/wiki/Zygnema
- Blepharisma Americana - (red) https://www.youtube.com/watch?v=te_dW1vi6Ts
- Blepharisma Japonicum - (red) https://en.wikipedia.org/wiki/Blepharisma_japonicum https://www.youtube.com/watch?v=5OB7kMCpDdM
- Hidra Vulgaris - (white) long straight form https://www.youtube.com/watch?v=vkrxgNjmTsk
- Alivibrio fischeri - (bioluminiscence) blue luminiscence https://en.wikipedia.org/wiki/Aliivibrio_fischeri
Here is a web page with information about some microorganism, how to cultivate them, the price of some of them.
MICROSCOPIC PHOTOGRAPHY.
The first step is to find a subject, in my case, I want to work with Polycephalum/physarum an allowed sample in the laboratory, with samples taking from the human body, could be like Escherichia Coli and also some other microorganism that can be found in the Ilm Park, in Weimar-Germany.
Types of photography with a microscope:
- Köhler Illumination: neutral and uniform backlighting, helps to maximize the contrast and resolution in the image.
- Darkfield: makes an object glow against a black background by using a darkfield condenser into the microscope (there are to types; dry or oil condenser).
Link to examples in microscopic photography & video work samples.
https://www.youtube.com/watch?v=olCEGsKWQ3c
https://www.popphoto.com/tips-pro-microscopic-photography
SELECTED SPECIMENS FURTHER INVESTIGATION
- This specimen is found globally in marine environments.
- Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, but are found in higher concentrations in symbiosis with certain deep-sea life like the normal gut microbiota of marine animals.
- The bioluminescence of A. fischeri is caused by transcription of the lux operon, which is induced through population-dependent quorum sensing. The population of A. fischeri needs to reach an optimal level to activate the lux operon and stimulate light production.
- Medium:
NaCl 30 g Glycerol 1 g Pepton (Bacxto-peptone) 10 g Meat extract 3 g Made up with distilled water to 1000 ml