Paola Stephania/LINK PROJECT 2

SELECTED SPECIMENS FURTHER INVESTIGATION

Polycephalum/physarum

- Wikipedia link: https://es.wikipedia.org/wiki/Physarum_polycephalum (general information around this specimen)

- TED: https://www.ted.com/speakers/heather_barnett_1 (a conference opening a window around experiments with this specimen)

- The Slime Mould Collective: http://slimoco.ning.com/

(here can be found experiments around the world around this specimen) really cool :) (personal opinion)

- Video educational video https://www.youtube.com/watch?v=40f7_93NIgA (Simon Garnier - assistant professor in NJIT talks about his studies)

- Slime Molds Remember - but Do they learn? https://www.quantamagazine.org/slime-molds-remember-but-do-they-learn-20180709/?fbclid=IwAR193K1ULzbxSq-BNUijJWsydeAbAHa6-Eq2sXZpdbLl9_ssvLel3Aph_GM " Most importantly, slime molds can be taught new tricks; depending on the species, they may not like caffeine, salt or strong light, but they can learn that no-go areas marked with these are not as bad as they seem, a process known as habituation." (This quote from this article, give me a hint, in order to experiment with salinity in the medium of the Polycephalum). Dussutour make an experiment placing oatmeal at the other side of a gelatine substance made with either caffeine or quinine in order to obligate the Polycephalum to cross, she learned that after the organism get used to a certain substance, they will cross to the food in this case in the same kein of medium that was located in the first time. (the Physarum have "memorie" channels

- Video testing intelligence of the specimen https://www.youtube.com/watch?v=czk4xgdhdY4

- Video Polycephalum in technology (computer storage) https://www.youtube.com/watch?v=HKZ2LtfDrmg

- This specimen is found in the forest and is a type of amoeba.

- Grows at a temperature of around 22° - 24°.

- Medium:

100ml of distilled water

2g of agar

Leaflets

Aliivibrio Fischeri

- Wikipedia link:

- This specimen is found globally in marine environments.

- Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, lives as a symbiont in the light organ of certain fish and squid species. Grows at temperatures of 24°.

- The bioluminescence of A. fischeri is caused by transcription of the lux operon, which is induced through population-dependent quorum sensing. The population of this specimen needs to reach an optimal level to activate the lux operon and stimulate light production.

- Many people have work with this specimen trying to make their own version of the medium. Common ingredients in most media include Tryptone, yeast extract, glycerol, and roughly 3% NaCl by weight.

- Medium:

NaCl 30 g

Glycerol 1 g

Pepton (Bacxto-peptone) 10 g

Meat extract 3 g

Made up with distilled water to 1000 ml

EXPERIMENTATION

• Day 1: The extraction of Alii Vibrio Fischeri is made, and a sample of "Polysephalum/physarum in a clean environment is made.

• Day 4: I failed to extract the Alii Vibrio Fischeri into the medium, after three days there was no blue glowing as seen in the first day inside the medium that I made.

The isolation of Polysephalum/physarum was correctly made, I will wait one week for it to grow in order to implant it in a salty medium simulating the natural environment of the Alii Vibrio Fischeri.