Paola Stephania/LINK PROJECT 2

SELECTED SPECIMENS FURTHER INVESTIGATION

Aliivibrio Fischeri

- This specimen is found globally in marine environments.

- Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, lives as a symbiont in the light organ of certain fish and squid species. Grows at temperatures of 24°.

- The bioluminescence of A. fischeri is caused by transcription of the lux operon, which is induced through population-dependent quorum sensing. The population of this specimen needs to reach an optimal level to activate the lux operon and stimulate light production.

- Many people have work with this specimen trying to make their own version of the medium. Common ingredients in most media include Tryptone, yeast extract, glycerol, and roughly 3% NaCl by weight

- Medium:

NaCl 30 g

Glycerol 1 g

Pepton (Bacxto-peptone) 10 g

Meat extract 3 g

Made up with distilled water to 1000 ml

Polycephalum/physarum

- This specimen is found in the forest.

- Grows at a temperature of around 22° - 24°.

- Medium:

100ml of distilled water

2g of agar

Leaflets

Alii Vibrio Fischeri

• Day 1: The extraction of Alii Vibrio Fischeri is made.

• Day