Paola Stephania/LINK PROJECT 1

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CULTIVATION OF Haematococcus Pluvialis.

- Known by its high content of the strong antioxidant astaxanthin.

* SCIENTIFIC CLASIFICATION Division: Chlorophyta Class: Chlorophyceae Order: Chlamydomonadales Family: Haematococcaceae Genus: Haematococcus Species: H. pluvialis Binomial name Haematococcus pluvialis

https://en.wikipedia.org/wiki/Haematococcus_pluvialis HCP 2.PNG

  • MEDIUM

- 100ml of water

- 3% of vinasse

- 0.7% of NaCl.

The pH is adjusted to 7.0. A 0.4 g/L quantity of inoculum can be used for the initial culture (cells in vegetative growth). The culture must be performed with 0.5 vvm air at 25°C, and until 15 days of culture.

Note: in the laboratory was no Vinasse, so another bioproduct of the sugar was used in order to prepare the medium Malz extract. (brennwert: 1.216kJ, fett 0.2g, kohlenhydrate 65g, Zucker 45g, Eiweiss 5g, Salz0.02g.)

  • Day 1: Three samples were made all with the same concentration of salinity but with different percentage of vinasse, and with the same PH 7. Preparation and from left to right; original medium, 10% of original medium diluted in 100ml of water, 1% of original sample diluted in 100ml of water.

Wd7.jpg Wd8.jpg

The three samples contain:

1 Sample 3,00g malz extract, 100ml water & 0,7g NACl. 2 Sample 0,30g malz extract, 100ml water & 0,7g NACl. 3 Sample 0,03g malz extract, 100ml water & 0,7g NACl.

  • Day 2: An observation under the microscope is made in order to see the specimen.

HCP 1.PNG

  • Day 4: After 4 days of growth the specimens:...