Line 8: Line 8:
 
[[File:wd12.JPG|400px]]
 
[[File:wd12.JPG|400px]]
  
After 5 days the samples of ''Polycephalum/physarum'' didn't come to live again, my first hypothesis is that the medium was too salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state).
+
* Day 5: After 5 days the samples of ''Polycephalum/physarum'' didn't come to live again, my first hypothesis is that the medium was too salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state).
  
 
[[File:wd23.jpg|400px]]
 
[[File:wd23.jpg|400px]]
  
* Day 2: I made a new sample with a new medium. Parallel to the samples that I use for the experiment I started to use this new Medium to grow the specimen (to have live healthy samples to experiment), I started from the formula that Fernanda Caicedo gets to in her previous experiments, and I altered for my special use.  
+
* Day 7: I made a new sample with a new medium. Parallel to the samples that I use for the experiment I started to use this new Medium to grow the specimen (to have live healthy samples to experiment), I started from the formula that Fernanda Caicedo gets to in her previous experiments, and I altered for my special use.  
  
 
Medium:
 
Medium:
Line 26: Line 26:
 
[[File:pp-1.jpeg|400px]]
 
[[File:pp-1.jpeg|400px]]
  
* Day 3: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non-salty medium and the one in the salty medium seems that have died.
+
* Day 8: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non-salty medium and the one in the salty medium seems that have died.
  
 
[[File:pp-2.jpg|400px]]
 
[[File:pp-2.jpg|400px]]
  
*Day 7: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have a red color.
+
*Day 12: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have a red color.
  
 
[[File:wd24.jpg|400px]]
 
[[File:wd24.jpg|400px]]
Line 40: Line 40:
 
[[File:wd26.jpg|400px]]
 
[[File:wd26.jpg|400px]]
  
*Day 8: A made some new medium ''(this time for the salinity medium I just used 0.2% of salinity concentration)'' and made a new experiment, this time in the divided medium I used just agar at the left and the salinity 0.2% concentration medium at left. Two control samples at left with the ideal medium for the growth of the specimen and at the right full concentration of salinity in all the Petri dish.
+
*Day 13: A made some new medium ''(this time for the salinity medium I just used 0.2% of salinity concentration)'' and made a new experiment, this time in the divided medium I used just agar at the left and the salinity 0.2% concentration medium at left. Two control samples at left with the ideal medium for the growth of the specimen and at the right full concentration of salinity in all the Petri dish.
  
 
[[File:wd27.jpg|400px]]
 
[[File:wd27.jpg|400px]]
Line 49: Line 49:
  
  
* Day 10: The living samples are turning pink again, and the samples in the salinity medium seem to be like they are dying again.
+
* Day 15: The living samples are turning pink again, and the samples in the salinity medium seem to be like they are dying again.
  
 
[[File:wd35.jpg|400px]]
 
[[File:wd35.jpg|400px]]
  
*Day 12: Definitely the samples in the salinity medium are death. Ant the contamination/chemical reaction continues in the other samples.
+
*Day 17: Definitely the samples in the salinity medium are death. Ant the contamination/chemical reaction continues in the other samples.
  
 
[[File:wd36.jpg|400px]]
 
[[File:wd36.jpg|400px]]
  
* Day 15: Because the salinity in the medium is too high for the specimens to growth, I reduced the salinity inside the medium into 0.03% in order to see the reaction of the specimen. The right samples continue to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium.
+
* Day 20: Because the salinity in the medium is too high for the specimens to growth, I reduced the salinity inside the medium into 0.03% in order to see the reaction of the specimen. The right samples continue to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium.
  
 
[[File:wd37.jpg|400px]]
 
[[File:wd37.jpg|400px]]
  
* Day 17: For the ideal medium the specimen is growing, but after a week it acquire some mode, the middle sample move to the food area away from the salinity medium, and the specimen in the salinity medium started to move in order to try to find food. It is tolerating the salinity medium. The specimen in the salinity medium with 0.03% concentration of NaCl is starting to get into hibernation mode, I put some food in order to make it strong before transplanting it into a new medium with higher concentrations of NaCl.
+
* Day 22: For the ideal medium the specimen is growing, but after a week it acquire some mode, the middle sample move to the food area away from the salinity medium, and the specimen in the salinity medium started to move in order to try to find food. It is tolerating the salinity medium. The specimen in the salinity medium with 0.03% concentration of NaCl is starting to get into hibernation mode, I put some food in order to make it strong before transplanting it into a new medium with higher concentrations of NaCl.
  
 
[[File:wd38.jpg|400px]]
 
[[File:wd38.jpg|400px]]
  
*Day 19: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination.  
+
*Day 24: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination.  
 
Because of health conditions and the use that can be given in the laboratory where I work, I put away these samples (disposed of them in a clean way).  
 
Because of health conditions and the use that can be given in the laboratory where I work, I put away these samples (disposed of them in a clean way).  
  
Line 77: Line 77:
  
  
*Day 20: I continue increasing the salinity quantities in the medium of the specimen to 0.04% and 0.05%. It is working.  
+
*Day 25: I continue increasing the salinity quantities in the medium of the specimen to 0.04% and 0.05%. It is working.  
 
Because of the vacation period, I put all my samples into rest (hibernation) and I will wake them up again in 3 weeks.
 
Because of the vacation period, I put all my samples into rest (hibernation) and I will wake them up again in 3 weeks.
  
 
[[File:wd48.jpg|400px]]
 
[[File:wd48.jpg|400px]]
  
*Day 45:
+
*Day 50:
 
I try without success to revive my trained samples of Physarum polycephalum. My hypothesis is that the medium where I tried to revive them was not the one with the salinity concentration where they were taught before that they will find food. Sadly I don't have more samples of the taught samples in 0.04% NaCl to prove my theory.  
 
I try without success to revive my trained samples of Physarum polycephalum. My hypothesis is that the medium where I tried to revive them was not the one with the salinity concentration where they were taught before that they will find food. Sadly I don't have more samples of the taught samples in 0.04% NaCl to prove my theory.  
  
 
[[File:wd50.jpg|400px]]
 
[[File:wd50.jpg|400px]]
  
*Day 53:
+
*Day 58:
 
I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and in order to start the test of adaptation again.
 
I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and in order to start the test of adaptation again.
  
Line 96: Line 96:
 
[[File:wd55.jpg|400px]]
 
[[File:wd55.jpg|400px]]
  
*Day 55:
+
*Day 60:
 
With these new healthy samples of the specimen, I made two Petri dishes in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium. I increase the salinity till 0.06% to test if the specimen is able to adapt to this salinity environment according to the results I receive from the first tests.
 
With these new healthy samples of the specimen, I made two Petri dishes in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium. I increase the salinity till 0.06% to test if the specimen is able to adapt to this salinity environment according to the results I receive from the first tests.
  
Line 102: Line 102:
 
[[File:wd52.jpg|400px]]
 
[[File:wd52.jpg|400px]]
  
*Day 57:
+
*Day 62:
 
After two days the specimen moved to the salinity 0.06% medium in order to find the food and is tolerating this environment/medium.  
 
After two days the specimen moved to the salinity 0.06% medium in order to find the food and is tolerating this environment/medium.  
  
Line 108: Line 108:
 
[[File:wd57.jpg|400px]]
 
[[File:wd57.jpg|400px]]
  
*Day 59:
+
*Day 64:
 
I create new Petri dishes the half is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 0.12% of salinity (there I situated the food).
 
and the other half is with 0.12% of salinity (there I situated the food).
Line 115: Line 115:
 
[[File:wd60.jpg|400px]]
 
[[File:wd60.jpg|400px]]
  
*Day 61:  
+
*Day 66:  
 
After two days the specimen moved to the salinity 0.12% medium in order to find the food and is tolerating this environment/medium.  
 
After two days the specimen moved to the salinity 0.12% medium in order to find the food and is tolerating this environment/medium.  
  
Line 121: Line 121:
 
[[File:wd62.jpg|400px]]
 
[[File:wd62.jpg|400px]]
  
*Day 63:  
+
*Day 68:  
 
I create new Petri dishes the half is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 0.24% of salinity (there I situated the food).  
 
and the other half is with 0.24% of salinity (there I situated the food).  
Line 129: Line 129:
 
[[File:wd64.jpg|400px]]
 
[[File:wd64.jpg|400px]]
  
*Day 65:  
+
*Day 70:  
 
I create new petri dishes the half is the previous environment with 0.24% of salinity (there I situated the Physarum polycephalum)  
 
I create new petri dishes the half is the previous environment with 0.24% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 0.3% of salinity (there I situated the food). One of the previous samples got contamination so I continue with just one sample.
 
and the other half is with 0.3% of salinity (there I situated the food). One of the previous samples got contamination so I continue with just one sample.
Line 135: Line 135:
 
[[File:wd66.jpg|400px]]
 
[[File:wd66.jpg|400px]]
  
*Day 67:  
+
*Day 72:  
 
After two days the specimen moved to the salinity 0.3% medium in order to find the food and is tolerating this environment/medium.  
 
After two days the specimen moved to the salinity 0.3% medium in order to find the food and is tolerating this environment/medium.  
  
 
[[File:wd65.jpg|400px]]
 
[[File:wd65.jpg|400px]]
  
*Day 69:  
+
*Day 74:  
 
I create new Petri dishes the half is the previous environment with 0.3% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 0.3% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 0.6% of salinity (there I situated the food).  
 
and the other half is with 0.6% of salinity (there I situated the food).  
Line 146: Line 146:
 
[[File:wd67.jpg|400px]]
 
[[File:wd67.jpg|400px]]
  
*Day 74:  
+
*Day 79:  
 
After two days the specimen hasn't moved to the salinity 0.6% medium, I give it 3 more days and after this time the specimen moved to the salinity 0.6% medium in order to find the food and is tolerating this environment/medium.  
 
After two days the specimen hasn't moved to the salinity 0.6% medium, I give it 3 more days and after this time the specimen moved to the salinity 0.6% medium in order to find the food and is tolerating this environment/medium.  
  
 
[[File:wd68.jpg|400px]]
 
[[File:wd68.jpg|400px]]
  
*Day 75:  
+
*Day 80:  
 
I create new Petri dishes the half is the previous environment with 0.6% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 0.6% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 1% salinity (there I situated the food).  
 
and the other half is with 1% salinity (there I situated the food).  
Line 157: Line 157:
 
[[File:wd69.jpg|400px]]
 
[[File:wd69.jpg|400px]]
  
*Day 76:  
+
*Day 81:  
 
After two days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium.  
 
After two days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium.  
  
 
[[File:wd70.jpg|400px]]
 
[[File:wd70.jpg|400px]]
  
*Day80:  
+
*Day 90:  
 
I create new Petri dishes the half is the previous environment with 1% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 1% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 2% salinity (there I situated the food).  
 
and the other half is with 2% salinity (there I situated the food).  
Line 168: Line 168:
 
[[File:wd71.jpg|400px]]
 
[[File:wd71.jpg|400px]]
  
*Day 86:  
+
*Day 91:  
 
After six days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium. The specimen is not growing to fast, so I will let it more time into the new salinity medium, in order for it to adapt.
 
After six days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium. The specimen is not growing to fast, so I will let it more time into the new salinity medium, in order for it to adapt.
  
 
[[File:wd72.jpg|400px]]
 
[[File:wd72.jpg|400px]]
  
*Day90:  
+
*Day 95:  
 
I create new Petri dishes the half is the previous environment with 2% of salinity (there I situated the Physarum polycephalum)  
 
I create new Petri dishes the half is the previous environment with 2% of salinity (there I situated the Physarum polycephalum)  
 
and the other half is with 3% salinity (there I situated the food).
 
and the other half is with 3% salinity (there I situated the food).
  
*Day 92:  
+
*Day 97:  
 
+
There was contamination in the lab and sadly many of my samples got contaminated, therefore I tried to create new ones in order to continue the experiment where it was left.
  
 
* Day 100: I translated the specimens into new Petri dishes with their new level of salinity in order to keep the specimens healthy.  
 
* Day 100: I translated the specimens into new Petri dishes with their new level of salinity in order to keep the specimens healthy.  

Revision as of 21:18, 25 March 2019

Experimentation with Polycephalum/physarum.

Here can be found the diary of work made with this specimen.


  • Day 1: In order to test if this specimen can grow in salty environments I made the following sample. In the right the two first specimens of Polycephalum/physarum are in the medium with; 0.5 gr Agar, 25ml water and 1 flake Leaflets. In the middle the medium is divided in the half, the right half has the previous medium and in the left, the medium is made with; 3% of NaCl (simulating the salinity of the sea), 0.5 gr Agar, 25ml water and 1 flake Leaflet is situated 1mm inside the salty environment. The left sample is made totally with the previous medium (the salty medium simulating the salinity of the sea).

Wd12.JPG

  • Day 5: After 5 days the samples of Polycephalum/physarum didn't come to live again, my first hypothesis is that the medium was too salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state).

Wd23.jpg

  • Day 7: I made a new sample with a new medium. Parallel to the samples that I use for the experiment I started to use this new Medium to grow the specimen (to have live healthy samples to experiment), I started from the formula that Fernanda Caicedo gets to in her previous experiments, and I altered for my special use.

Medium:

  • 100 ml of water
  • 4 gr of cooked potatoes
  • 4 gr of oat flakes
  • (mix together)
  • 2 gr of agar
  • (mix all together and boil)

At left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.

Pp-1.jpeg

  • Day 8: After one night, the sample in the normal environment is growing normally, the sample in the half mediums moved to the non-salty medium and the one in the salty medium seems that have died.

Pp-2.jpg

  • Day 12: After 10 days the specimen in the normal conditions environment is trying to find new food, is moving because of some contamination inside the Petri dish, the sample with the half environments didn't growth well, and it seems to be some kind of mutation/contamination/reaction of red color, this event also happened with the samples that I'm growing in order to use in these experiments. The specimens in the salty medium died, but one of the samples have a red color.

Wd24.jpg

NOTE: Samples with the pink/red contamination/mutation/reaction. Other samples that I'm growing using the same made medium and the same original Petri dish with the specimen doesn't have this situation. I don't have an explanation for this phenomena at the moment.

Wd25.jpg

Wd26.jpg

  • Day 13: A made some new medium (this time for the salinity medium I just used 0.2% of salinity concentration) and made a new experiment, this time in the divided medium I used just agar at the left and the salinity 0.2% concentration medium at left. Two control samples at left with the ideal medium for the growth of the specimen and at the right full concentration of salinity in all the Petri dish.

Wd27.jpg

After one night the specimen in the ideal medium is already growing, the specimen in the middle medium is not moving like the last time (probably because this time the food is concentrated in the salinity medium) and the samples in the salinity medium seem to be dying again.

Wd28.jpg


  • Day 15: The living samples are turning pink again, and the samples in the salinity medium seem to be like they are dying again.

Wd35.jpg

  • Day 17: Definitely the samples in the salinity medium are death. Ant the contamination/chemical reaction continues in the other samples.

Wd36.jpg

  • Day 20: Because the salinity in the medium is too high for the specimens to growth, I reduced the salinity inside the medium into 0.03% in order to see the reaction of the specimen. The right samples continue to be just a control sample with an ideal medium and the medium in the middle is a medium in between with food in the none salinity medium.

Wd37.jpg

  • Day 22: For the ideal medium the specimen is growing, but after a week it acquire some mode, the middle sample move to the food area away from the salinity medium, and the specimen in the salinity medium started to move in order to try to find food. It is tolerating the salinity medium. The specimen in the salinity medium with 0.03% concentration of NaCl is starting to get into hibernation mode, I put some food in order to make it strong before transplanting it into a new medium with higher concentrations of NaCl.

Wd38.jpg

  • Day 24: I made an observation under the microscope, the pink color in my samples is a bacteria, contamination that comes from the first petri dish that I used to extract a clean sample. Here can be found more pictures of the contamination.

Because of health conditions and the use that can be given in the laboratory where I work, I put away these samples (disposed of them in a clean way).


  • Day 25: I continue increasing the salinity quantities in the medium of the specimen to 0.04% and 0.05%. It is working.

Because of the vacation period, I put all my samples into rest (hibernation) and I will wake them up again in 3 weeks.

Wd48.jpg

  • Day 50:

I try without success to revive my trained samples of Physarum polycephalum. My hypothesis is that the medium where I tried to revive them was not the one with the salinity concentration where they were taught before that they will find food. Sadly I don't have more samples of the taught samples in 0.04% NaCl to prove my theory.

Wd50.jpg

  • Day 58:

I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and in order to start the test of adaptation again.

Wd53.jpg

Wd54.jpg

Wd55.jpg

  • Day 60:

With these new healthy samples of the specimen, I made two Petri dishes in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium. I increase the salinity till 0.06% to test if the specimen is able to adapt to this salinity environment according to the results I receive from the first tests.

Wd51.jpg Wd52.jpg

  • Day 62:

After two days the specimen moved to the salinity 0.06% medium in order to find the food and is tolerating this environment/medium.

Wd56.jpg Wd57.jpg

  • Day 64:

I create new Petri dishes the half is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum) and the other half is with 0.12% of salinity (there I situated the food).

Wd59.jpg Wd60.jpg

  • Day 66:

After two days the specimen moved to the salinity 0.12% medium in order to find the food and is tolerating this environment/medium.

Wd61.jpg Wd62.jpg

  • Day 68:

I create new Petri dishes the half is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum) and the other half is with 0.24% of salinity (there I situated the food).

Wd63.jpg

Wd64.jpg

  • Day 70:

I create new petri dishes the half is the previous environment with 0.24% of salinity (there I situated the Physarum polycephalum) and the other half is with 0.3% of salinity (there I situated the food). One of the previous samples got contamination so I continue with just one sample.

Wd66.jpg

  • Day 72:

After two days the specimen moved to the salinity 0.3% medium in order to find the food and is tolerating this environment/medium.

Wd65.jpg

  • Day 74:

I create new Petri dishes the half is the previous environment with 0.3% of salinity (there I situated the Physarum polycephalum) and the other half is with 0.6% of salinity (there I situated the food).

Wd67.jpg

  • Day 79:

After two days the specimen hasn't moved to the salinity 0.6% medium, I give it 3 more days and after this time the specimen moved to the salinity 0.6% medium in order to find the food and is tolerating this environment/medium.

Wd68.jpg

  • Day 80:

I create new Petri dishes the half is the previous environment with 0.6% of salinity (there I situated the Physarum polycephalum) and the other half is with 1% salinity (there I situated the food).

Wd69.jpg

  • Day 81:

After two days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium.

Wd70.jpg

  • Day 90:

I create new Petri dishes the half is the previous environment with 1% of salinity (there I situated the Physarum polycephalum) and the other half is with 2% salinity (there I situated the food).

Wd71.jpg

  • Day 91:

After six days the specimen moved to the salinity 1% medium in order to find the food and is tolerating this environment/medium. The specimen is not growing to fast, so I will let it more time into the new salinity medium, in order for it to adapt.

Wd72.jpg

  • Day 95:

I create new Petri dishes the half is the previous environment with 2% of salinity (there I situated the Physarum polycephalum) and the other half is with 3% salinity (there I situated the food).

  • Day 97:

There was contamination in the lab and sadly many of my samples got contaminated, therefore I tried to create new ones in order to continue the experiment where it was left.

  • Day 100: I translated the specimens into new Petri dishes with their new level of salinity in order to keep the specimens healthy.

Salinity Percentage mediums