The original idea was to see if there was any relation between Alii Vibrio fischeri (a specimen that life in warm around 20°C oceans) & Physarum Polycephalum (a specimen that life's in the forest in warm temperatures 20°C). In order to test this, I made an investigation around the two specimens. The second step was to cultivate both of them, but the extraction of the first specimen from an octopus didn't give results, so I redirected my project into teaching Physarum Polycephalum to adapt to salinity environments.
INVESTIGATION OF THE SPECIMENS
- Wikipedia link: https://en.wikipedia.org/wiki/Physarum_polycephalum (general information around this specimen)
- TED: https://www.ted.com/speakers/heather_barnett_1 (a conference opening a window around experiments with this specimen)
- The Slime Mould Collective: http://slimoco.ning.com/
(here can be found experiments around the world around this specimen) really cool :) (personal opinion)
- Video educational video https://www.youtube.com/watch?v=40f7_93NIgA (Simon Garnier - assistant professor in NJIT talks about his studies)
- Slime Molds Remember - but Do they learn? https://www.quantamagazine.org/slime-molds-remember-but-do-they-learn-20180709/?fbclid=IwAR193K1ULzbxSq-BNUijJWsydeAbAHa6-Eq2sXZpdbLl9_ssvLel3Aph_GM " Most importantly, slime molds can be taught new tricks; depending on the species, they may not like caffeine, salt or strong light, but they can learn that no-go areas marked with these are not as bad as they seem, a process known as habituation." (This quote from this article, give me a hint, in order to experiment with salinity in the medium of the Polycephalum). Dussutour make an experiment placing oatmeal at the other side of a gelatine substance made with either caffeine or quinine in order to obligate the Polycephalum to cross, she learned that after the organism get used to a certain substance, they will cross to the food in this case in the same kind of medium that was located in the first time. (the Physarum have "memory" channels). She made also an experiment testing salinity environments, was the specimen adapted himself to this environment, (also she tested with her team if this information could be communicated to other samples that were not "trained" to this kind of environment).
- Video testing intelligence of the specimen https://www.youtube.com/watch?v=czk4xgdhdY4
- Video Polycephalum in technology (computer storage) https://www.youtube.com/watch?v=HKZ2LtfDrmg
- This specimen is found in the forest and is a type of amoeba.
- Grows at a temperature of around 22° - 24°.
- Wikipedia link:
- This specimen is found globally in marine environments.
- Is found in very low quantities in almost all oceans of the world in temperate or subtropical water, lives as a symbiont in the light organ of certain fish and squid species. Grows at temperatures of 24°.
- The bioluminescence of A. fischeri is caused by transcription of the lux operon, which is induced through population-dependent quorum sensing. The population of this specimen needs to reach an optimal level to activate the lux operon and stimulate light production.
- Many people have work with this specimen trying to make their own version of the medium. Common ingredients in most media include Tryptone, yeast extract, glycerol, and roughly 3% NaCl by weight.
EXPERIMENTATION with Alii Vibrio Fischeri
CONCLUSIONS: Because I failed in the extraction of the Aliivibrio Fischeri, I will start with the cultivation of Polycephalum/physarum in order to confirm if this specimen can be cultivated in salty environments, and more specifically if it can survive in a 3% concentration of NaCl medium. According to the previous investigations of Dussutour, it is possible for it to survive in salinity environments, so I will explore this idea.
EXPERIMENTATION with Polycephalum/physarum
After 5 days the samples of Polycephalum/physarum didn't came to live again, my first hypothesis is that the medium was to salty my second is that the specimen needs more time in order to come to live again (awake from hibernation state).
At left a normal medium, at the center a sample half with the normal medium and the other half with 3% of NaCl, and the third sample with just the salty sample.
NOTE: Samples with the pink/red contamination/mutation/reaction. Other samples that I'm growing using the same made medium and the same original Petri dish with the specimen don't have this situation. I don't have an explanation for this fenomena in the moment.
After one night the specimen in the ideal medium is already growing, the specimen in the middle medium is not moving like the last time (probably because this time the food is concentrated in the salinity medium) and the samples in the salinity medium seem to be dying again.
Because of health conditions and the use that can be given in the laboratory where I work, I put away this samples (disposed of them in a clean way).
Because of the vacation period, I put all my samples into rest (hibernation) and I will wake them up again in 3 weeks.
I try without success to revive my trained samples of Physarum polycephalum. My hypothesis is that the medium where I tried to revive them was not the one with the salinity concentration where they were taught before that they will find food. Sadly I don't have more samples of the taught samples in .04Nacl to prove my theory.
I cultivate new polycephalum taken from samples of Niilo (A-#), Julian (B-#), Antje(C-#), and in order to start the test of adaptation again.
With these new healthy samples of the specimen, I made two petri disches in order to reeducate Physarum Policephalum to live in salinity environments. To do that I made a neutral medium (100ml of water + 2 gr of agar) and a medium with 0.06% of salinity (100ml of water + 2 gr of agar + 0.06 gr of NaCl) I put the Pysarum Policephalum in the neutral medium and the food in the salinity medium.
After two days the specimen moved to the salinity medium in order to find the food and is tolerating this environment/medium. (n = neutral medium, # = percentage of salinity)
I create new petri disches the halb is the previous environment with 0.06% of salinity (there I situated the Physarum polycephalum) and the other halb is with 0.12% of salinity (there I situated the food).
After two days the specimen moved to the salinity 0.121% medium in order to find the food and is tolerating this environment/medium.
I create new petri disches the halb is the previous environment with 0.12% of salinity (there I situated the Physarum polycephalum) and the other halb is with 0.24% of salinity (there I situated the food).
I create new petri disches the halb is the previous environment with 0.24% of salinity (there I situated the Physarum polycephalum) and the other halb is with 0.3% of salinity (there I situated the food). One of the previous samples got contamination so I continue with just one sample.
After two days the specimen moved to the salinity 0.3% medium in order to find the food and is tolerating this environment/medium.
During the time that I adopted this specimen, I made many tests in order to get to know and understand the specimen. I'm also taking the class of "DIY Bio; doing things with biology", where I made many tests in terms of food, temperature, and light. This information can be found on the following websites:
After making the salinity experiment with the Physarum Polycephalum, I get into the conclusion that this specimen can learn to survive in mediums that have salt concentrations. It can learn to survive in them in a process of increasing the salinity, but not if it is situated in a salinity medium without previous teaching.