Sun. 31.05 Starting to notice this white-ish slime. First I thought it was mold but now I think it is the trace that Physarum leaves behind itself - which if it has enough space hinders it from going to this spot again. Might be having a strange reaction with the Agar?
Sun. 31.05 Seeing an answer to the question: does physarum grow on surfaces that are not Agar: yes! Does it maybe draw the moisture from the surface of agar so that it doesn't need it in other/all areas?
Also you can start to see the digested oats that are no longer yellow so the blob moved off of them because they offer no more for it.
Sat. 30.05 - decided to 'feed' some less to start seeing a difference in behaviour
Sun. 31.05 - because I don't want to much moisture to build up (might lead to bacterial mold) and to keep caring well for the Physarum & first get a better understanding of it, I gave each dish a full spoon of oats today.
The Physarum's structure is obviously much better visible when NOT ON OATS - so what are aesthetic and sustainable ways to replace the oats or make the food source disappear?
Mon. 01.06 - The first petri-dish, probably older than a week now changed colors, it doesn't look like mold but it's clearly much much darker then if was yesterday. Also doesn't look like the other phases of the life cycle?
Mon. 01.06 - Also there is a lot more moisture in the dishes again - maybe today is finally a day to inoculate? Clearly 6 days is the limit - better to change them after 5 days - like this guide also suggests: https://drive.google.com/drive/folders/1BYdpvPxnUNgdqqu1ELHTnbWlc0cQqvu6
Mon. 01.06 - Here you can see Physarum build a squiggly mass on top of the oats, it's more like a pile of yellow little blobs than the vein like structure.
Mon. 01.06 - Also other dishes are starting to form black dots which I can see more clearly from the bottom - which show bacterial/Yeast contamination and indicate that I should change the dish. Clearly the Physarum wants out, but isen't even healthy enough to make it.
A new inoculation follows!
What isen't really clear to me is what to do with the old dishes from which I inoculate new ones? Do I let them go their course? Do I try to transfer as much of the blob and then let the leftovers dry?
Answer - Mon. 08.06: I wait until the dish is fully dried out our possibly contaminated to throw away whole dish in worst case or clean out the already dried Physarum. I now have pre-made petri-dishes for when a lot tries to escape. If only a little tries to come out I put it back in the same dish.
specific documentation of Physarum in captivity: https://kjakubek.hotglue.me/?iforganismdocumentation/
online Processbook in process: https://kjakubek.hotglue.me/?Iforganism