GMU:DIY Bio: doing things with biology/Sirin Unmanee/Growing Bacteria for my Project
Bacteria
In this document, I attempt to capture the shape of each bacteria that I'm cultivating, using them as a reference for my project[1] Then, I will generate graphic visuals from their electric capacity, based on their form.
08.12.2018 - 11:30 Bacteria from Kombucha with Gluconcetobacter medium under the microscope
08.12.2018 - 11:30 Bacteria from Kombucha with LBP medium under the microscope
Also, I accidentally grew a white mold from a Kombucha bacteria and I want to include it to my project.
16.01.2019 - 13:30 White mold from Kombucha bacteria
20.01.2019 - 12:30 White mold from Kombucha bacteria
Then, I worked with bacteria form dialy life stuffs, smartphone and doorknob. The result is interesting, because they produced a different form even I cultivated them with the same medium. The bacteria from smartphone formed tiny yellow/orange dots, which is quite the same as a yellow bacteria form Kombucha, while the bacteria from door knob formed diffuse white dots all over the medium's surface.
So, I searched for the information about different types of bacteria due to its color, then found out that the yellow/orange bacteria is probably called Staphylococcus Citreus and the white is probably called Staphylococcus Epidermidis, however, I'm not so sure since I just only compared my bacteria with the shown example on the website.
In bacteria, pigment formation is associated with morphological characteristics, cellular activities, pathogenesis, protection and survival.
Pigments are produced by bacteria to absorb UV radiation or to quench oxygen free radicals. In both the cases bacterial pigment play important role of the cell protection. Some bacterial pigments are antibiotics which are active against phytopathogenic fungi, bacteria, and yeasts; also active against human pathogenic Gram positive or negative bacteria and fungi.
source : [2]
02.01.2019 - 13:30 Bacteria from a smartphone with LBP medium
02.01.2019 - 13:30 Bacteria from a door knob with LBP medium
After this result, I attempted to grow them again with the same method to test that is there a different outcome. This time, they both formed diffuse white dots, but still, the bacteria from the phone had yellow/orange dots.
16.01.2019 - 17:30 Bacteria from a smart phone and a door knob with LBP medium
After five days, the orange bacteria colonial is getting more discernible.
20.01.2019 - 12:30 Bacteria from a smart phone and a door knob with LBP medium
Fungus
Moreover, I worked with fungus, which are mold from a bread and a mushroom. I used a simple Agar medium but with a little portion of yeast.
I left my bread for about 3 weeks until it is completely covered by mold. At the beginning, I only expected the green mold (Penicillium) which can be normally seen, but there is also a yellow mold.
The type of mold that most commonly appears as yellow is Aspergillus, or it is just a different stage of the Penicillium.
16.01.2019 - 17:30 yellow and green mold from a bread
After five days, the mold changed its color and they formed different configurations. In the yellow mold's petri dish, appears the bigger mold's figures with yellow, dark yellow, green and white, while the mold's shape is more diffuse and smaller in the green mold's petri dish. Also, there are orange molds in the latter one.
20.01.2019 - 12:30 yellow and green mold from a bread