GMU:DIY Bio: doing things with biology/Paola Stephania Calderón/Final guide

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PROJECT IDEA 2

The first approximation is to recreated and find a specific patron in order to cultivate a specimen with a coral shape. The project idea is to experiment with the kombucha isolation method made before, in order to duplicate the results obtained with the coral shaped specimen so I can replicate and create a method to extract/isolate this specific specimen. The final project consist in creating a book/guide that people can use in order to replicate the experiment that I made in the laboratory.

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INVESTIGATION

Gluconacetobacter: https://en.wikipedia.org/wiki/Gluconacetobacter

EXPERIMENT APROXIMATION

- In order to do this, I will retake my previous results obtained in the first attempt to isolate a specimen from kombucha. This investigation can be seen in the following link [1]

- Medium: Gluconacetobacter (500ml)

Glucose - 10g

Peptone - 2.5g

Yeast extract - 2.5g

Na2HPO4 - 1.35g

Citric acid - 0.75g

Distilled or tap water - 500ml

Agar - 7.5g


POSIBLE SPECIMEN

Clonostachys sp.

Fusarium sp.


Acremonium masseei

Acremonium murorum

Talaromyces minioluteus


https://pdfs.semanticscholar.org/4d16/1cea571a604af97d9b05f23a3127eab92a83.pdf https://www.interdisciplinary-laboratory.hu-berlin.de/de/content/architektur-und-morphogenese-von-biofilmen/

Gluconacetobacter aggeris Gluconacetobacter asukensis Gluconacetobacter azotocaptans Gluconacetobacter diazotrophicus Gluconacetobacter entanii Gluconacetobacter europaeus Gluconacetobacter hansenii Gluconacetobacter intermedius Gluconacetobacter johannae Gluconacetobacter kakiaceti Gluconacetobacter kombuchae Gluconacetobacter liquefaciens (type species of the genus) Gluconacetobacter maltaceti Gluconacetobacter medellinensis Gluconacetobacter nataicola Gluconacetobacter oboediens Gluconacetobacter rhaeticus Gluconacetobacter sacchari Gluconacetobacter saccharivorans Gluconacetobacter sucrofermentans Gluconacetobacter swingsii Gluconacetobacter takamatsuzukensis Gluconacetobacter tumulicola Gluconacetobacter tumulisoli Gluconacetobacter xylinus